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微孔/FCCH025142 | Flowcell等™ 细胞周期检查点H2A.X DNA损伤试剂盒/FCCH025142/25测试和#47；配套元件
微孔/FCCH025142 | Flowcell等™ 细胞周期检查点H2A.X DNA损伤试剂盒/FCCH025142/25测试和#47；配套元件
商品编号：
FCCH025142
品牌：
密理博
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | FCCH025142 |
| TradeName |
|
| Description | FlowCellect™CellCycleCheckpointH2A.XDNADamageKit |
| Overview | Millipore’sFlowCellect™DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoidentifythelinkagebetweenDNAdamageinrelationtocellcyclecheckpointsinflowcytometryapplications.ThekitincludesadirectlyconjugatedAnti-phospho-HistoneH2A.X(Ser139)AlexaFluor®488antibodyplusaDNAdye(PropidiumIodide)whichallowstheresearchertoperformbivariateanalysistoidentifywhereDNAdamagetakesplacerelativetothepositioningwithinthecellcycle.Thekitincludesanoptimizedprotocolwithallofthenecessarycomponentstoprovidetheresearcheratrue“plugandplay”designfortheirresearchwhenstudyingDNAdamageandcellcyclecheckpoints.Thisimmunocytochemicalmethodisverysensitive,anditoffersanassaytoscreenanti-tumordrugsateveryphaseofthecellcycle. Inall,acomprehensiveunderstandingofDNAdamageanditsassociationwithcellcyclebehaviorcanprovidetheresearcherwithusefulinformationwhichwillbeimportantininterpretingtheintrinsicnatureofcellproliferation,apoptosis,DNArepair,andassistinthedevelopmentofanti-neoplasticagents.Millipore’sFlowCellect™DNADamage(HistoneH2A.X)andCellCycleAnalysisKitisdesignedtoallowtheresearchertoeasilyidentify,discriminate,andquantitatecellsundergoingDNAdamagerelativetoitspositioningwithinthecellcycle. AllFlowCellectkitsareoptimizedonguava®benchtopflowcytometers.FlowCellectkitscanbeusedonanyflowcytometerfollowingthesameprotocolprovidingresearchersareliableandfullyvalidatedsolutiontostudyDSBsandcellcycledistributionrightinthecomfortoftheirownlab.Thiskitcontainsoptimizedfixation,permeABIlization,wash,andflowbufferstoprovideresearcherswithacompletesolutionforbivariateanalysistoassessDNAdamageinrelationtopositioningwithinthecellcycle. |
| AlternateNames |
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| BackgroundInformation | DetectionofDNAdamageduetodoublestrandedbreaks(DSBs)isafundamentalmethodforassessingcellhealthandevaluationofanti-neoplasticagents.BivariateanalysismonitoringtheextentofH2A.XphosphorylationalongwithaDNAdyeoffersasensitiveandvaluabletoolinstudyingthefactorsthatinduceDNAdamage(measuredbythelevelofDSBs)and/oraffectDNArepair.UnderstandingwherethefirstindicationofDSBstakeplaceduringthecellcyclecanhelpresearchersunderstandthemechanismsinvolvedincellcyclearrestandDNArepairatcriticalcellcyclecheckpoints.MalfunctionsinthecellcycleinresponsetoDNAdamagecanincreasetheincidenceofcancersincetheDNArepairmachineryfailstooperateproperly.ThisisimportantbecausethecellhasseveralsystemsforinterruptingthecellcycleiftheDNArepairmachinerymalfunctions.EvidencesuggeststhatH2A.XphosphorylationmayrecruitcheckpointproteinstositesofDNAdamageandplayaroleinDNArepair. VariousfactorscancauseDNAdoublestrandedbreaksresultinginDNAdamage.PhysicalfactorssuchasUVandionizingrADIation,orchemicalfactorssuchassyntheticcompounds(anti-tumordrugs)canallcauseDNAdamage.RegardlessofthesourcetoinduceDNAdamage,theendresultwilleithercausecellcyclecheckpointarrest,apoptosis,orwilltriggertheDNArepairresponsetodamage. |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Fluorescent |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 4-8°Cforantibodiesandbuffers |
| BIOLOGicalInformation | |
|---|---|
| Clone | JBW301 |
| Host | Mouse |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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