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Millipore/17-10048 | ChIPAb+组蛋白H2A.Z-芯片验证抗体和引物集/17-10048/25分析每套25次分析。
Millipore/17-10048 | ChIPAb+组蛋白H2A.Z-芯片验证抗体和引物集/17-10048/25分析每套25次分析。
市场价：
¥8000.00
美元价：
4800.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-10048 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | ChIPAb+HistoneH2A.Z-ChIPValidatedAntibodyandPrimerSet |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HistoneH2A.ZsetincludestheHistoneH2A.Zantibody,anegativecontrolantibody(normalsheepIgG),andqPCRprimerswhichamplifya166bpregionofhumanGAPDH.TheHistoneH2A.Zandnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2A.Z-associatedchromatin. |
| AlternateNames |
|
| BackgroundInformation | H2A.Z,alsoknownasH2AFZ,isavariantofhistoneH2Avariantthatishighlyconservedfromyeasttohumans.Likeotherhistonevariants,H2A.Zisencodedbyauniquegene,resultinginaslightlydivergenttypeofH2Awithspecializedfunctions.H2A.ZisinvolvedinchromatincompactionbystABIlizingthehistoneoctamerwithinthenucleosome.Itpromotesgeneexpressionthroughthecreationofanopendomainofchromatinthatismoreeasilytranscribed.H2A.Zisnonrandomlydistributedthroughoutthegenome,targetedprimarilytopericentricheterochromatin,butexcludedfromtheinactiveXchromosomeandthenucleolus.H2A.Zisassociatedwithbotheuchromatinandfacultativeheterochromatin.DNAmethylationcaninfluencechromatinstructureandeffectgenesilencingbyexcludingH2A.Z,andlikewise,H2A.ZprotectsgenesfromDNAmethylation.Thus,H2A.Zplaysaroleinregulatingheterochromatinsilencingandisinvolvedintranscriptionalcontrol. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
|
| Presentation | Anti-HistoneH2A.Z(SheeppolyclonalIgG).Onevialcontaining25μgofaffinitypurifiedsheeppolyclonalIgGinPBS,pH7.4containing1%BSAwith0.02%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C. NormalSheepIgG.Onevialcontaining25µgofproteinGpurifiedsheepIgGin0.02Mpotassiumphosphate,0.15MNaCl,pH7.2,with0.01%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C. ControlPrimers.Onevialcontaining75μLof5μMofeachprimerspecificforhumanGAPDH.Storeat-20°C. FOR:TACTAGCGGTTTTACGGGCG REV:TCGAACAGGAGGAGCAGAGAGCGA |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BiologicalInformation | |
|---|---|
| Immunogen | LinearpeptidecorrespondingtheC-terminalaminoacids113-127ofhumanHistoneH2A.Z. |
| Epitope | C-terminus |
| Host | Sheep |
| Specificity | RecognizesHistoneH2A.Z,Mr~15kDa. |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.Widespeciesreactivityisexpected. Predictedtocross-reactwithzebrafish,mouse,chicken,rat,rabbit,andXenopusbasedonsequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.Severaltranscriptvariantsencodingdifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq]. |
| GeneSymbol |
|
| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Maybeinvolvedintheformationofconstitutiveheterochromatin.Mayberequiredforchromosomesegregationduringcelldivision.Ref.7 SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.H2AoritsvariantH2AFZformsanheterodimerwithH2B.H2AFZinteractswithINCENPBysimilarity. SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-122givesaspecifictagforepigenetictranscriptionalrepression. AcetylatedonLys-5,Lys-8andLys-12duringinterphase.AcetylationdisappearsatmitosisBysimilarity. NotphosphorylatedBysimilarity. SIMILARITY:BelongstothehistoneH2Afamily. MOLECULARWEIGHT:is13413.4Dafrompositions2-128.DeterminedbyESI.Monoisotopic,notmodified.Ref.8Ref.10Ref.11 |
| MolecularWeight | ~15kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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