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Millipore/MABE171 |抗磷酸化组蛋白H2A.X(Thr120)抗体,克隆11F5.3/MABE171/100µ;G
Millipore/MABE171 |抗磷酸化组蛋白H2A.X(Thr120)抗体,克隆11F5.3/MABE171/100µ;G
市场价:
¥0.00
美元价:
0.00
产品分类:
功能性抗体
公司分类:
Functional_antibody
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | |
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CatalogueNumber | MABE171 |
Description | Anti-phospho-HistoneH2A.X(Thr120)Antibody,clone11F5.3 |
AlternateNames |
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BackgroundInformation | HistoneH2A.XisavariantofhistoneH2A,andissimilarlyassociatedwithgenomicDNA.However,histoneisstructurallydifferentfromothermembersoftheH2AfamilyinpossessingaC-terminaltailthatcontainstheSer139residuethatisphosphorylatedinresponsetobreaksindouble-strandedDNA.ThephosphorylationofH2A.XisarapidprocessthatismediatedbyATM/ATRproteins. |
ProductInformation | |
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Format | Purified |
Control |
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Presentation | PurifiedmousemonoclonalIgG3κinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
StorageandShippingInformation | |
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StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
BIOLOGicalInformation | |
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Immunogen | KLH-conjugatedlinearpeptidecorrespondingtoHistoneH2A.XphosphorylatedatThr120. |
Epitope | PhosphorylatedThr120 |
Clone | 11F5.3 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Mouse |
Specificity | ThisantibodyrecognizesHistoneH2A.XphosphorylatedatThr120. |
SpeciesReactivity |
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AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneencodesamemberofthehistoneH2Afamily,andgeneratestwotranscriptsthroughtheuseoftheconservedstem-loopterminationmotif,andthepolyAadditionmotif. |
GeneSymbol |
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Modifications |
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PurificationMethod | ProteinGPurified |
UniProtNumber | |
UniProtSummary | FUNCTION:VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Requiredforcheckpoint-mediatedarrestofcellcycleprogressioninresponsetolowdosesofionizingrADIationandforefficientrepairofDNAdoublestrandbreaks(DSBs)specificallywhenmodifiedbyC-terminalphosphorylation. SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.InteractswithnumerousproteinsrequiredforDNAdamagesignalingandrepairwhenphosphorylatedonSer-140.TheseincludeMDC1,TP53BP1,BRCA1andtheMRNcomplex,composedofMRE11A,RAD50,andNBN.InteractionwiththeMRNcomplexismediatedatleastinpartbyNBN.AlsointeractswithDHX9/NDHIIwhenphosphorylatedonSer-140.InteractswithARRB2;theinteractionisdetectedinthenucleusuponOR1D2stimulation. SUBCELLULARLOCATION:Nucleus.Chromosome. DEVELOPMENTSTAGE:SynthesizedinG1aswellasinS-phase. DOMAIN:The[ST]-QmotifconstitutesarecognitionsequenceforkinasesfromthePI3/PI4-kinasefamily. POST-TRANSLATIONALMODIFICATION:PhosphorylatedonSer-140(toformgamma-H2AFXorH2AX139ph)inresponsetoDNAdoublestrandbreaks(DSBs)generatedbyexogenousgenotoxicagentsandbystalledreplicationforks,andmayalsooccurduringmeioticrecombinationeventsandimmunoglobulinclassswitchinginlymphocytes.PhosphorylationcanextenduptoseveralthousandnucleosomesfromtheactualsiteoftheDSBandmaymarkthesurroundingchromatinforrecruitmentofproteinsrequiredforDNAdamagesignalingandrepair.Widespreadphosphorylationmayalsoservetoamplifythedamagesignaloraidrepairofpersistentlesions.PhosphorylationofSer-140(H2AX139ph)inresponsetoionizingradiationismediatedbybothATMandPRKDCwhiledefectsinDNAreplicationinduceSer-140phosphorylation(H2AX139ph)subsequenttoactivationofATRandPRKDC.DephosphorylationofSer-140byPP2AisrequiredforDNADSBrepair.Inmeiosis,Ser-140phosphorylation(H2AX139ph)mayoccuratsynaptonemalcomplexesduringleptoteneasanATM-dependentresponsetotheformationofprogrammedDSBsbySPO11.Ser-140phosphorylation(H2AX139ph)maysubsequentlyoccursatunsynapsedregionsofbothautosomesandtheXYbivalentduringzygotene,downstreamofATRandBRCA1activation.Ser-140phosphorylation(H2AX139ph)mayalsoberequiredfortranscriptionalrepressionofunsynapsedchromatinandmeioticsexchromosomeinactivation(MSCI),wherebytheXandYchromosomescondenseinpachytenetoformtheheterochromaticXY-body.Duringimmunoglobulinclassswitchrecombinationinlymphocytes,Ser-140phosphorylation(H2AX139ph)mayoccuratsitesofDNA-recombinationsubsequenttoactivationoftheactivation-inducedcytidinedeaminaseAICDA.PhosphorylationatTyr-143(H2AXY142ph)byBAZ1B/WSTFdeterminestherelativerecruitmentofeitherDNArepairorpro-apoptoticfactors.PhosphorylationatTyr-143(H2AXY142ph)favorstherecruitmentofAPBB1/FE65andpro-apoptosisfactorssuchasMAPK8/JNK1,triggeringapoptosis.Incontrast,dephosphorylationofTyr-143byEYAproteins(EYA1,EYA2,EYA3orEYA4)favorstherecruitmentofMDC1-containingDNArepaircomplexestothetailofphosphorylatedSer-140(H2AX139ph).MonoubiquitinationofLys-120(H2AXK119ub)byRING1andRNF2/RING2complexgivesaspecifictagforepigenetictranscriptionalrepression.FollowingDNAdouble-strandbreaks(DSBs),itisubiquitinatedthrough"Lys-63"linkageofubiquitinmoietiesbytheE2ligaseUBE2NandtheE3ligasesRNF8andRNF168,leadingtotherecruitmentofrepairproteinstositesofDNAdamage.Monoubiquitinationandionizingradiation-induced"Lys-63"-linkedubiquitinationaredistinctevents.AcetylationatLys-37increasesinSandG2phases.ThismodificationhasbeenproposedtoplayaroleinDNAdouble-strandbreakrepair. SEQUENCESIMILARITIES:BelongstothehistoneH2Afamily. |
MolecularWeight | ~17kDaobserved |
PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。
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