Millipore/07-770 |抗乙酰组蛋白H2A.Z（Lys8）抗体| 07-770/07-770/100µ；G
市场价：
¥8840.00
美元价：
5304.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-770 |
| Description | Anti-acetyl-HistoneH2A.Z(Lys8)Antibody|07-770 |
| AlternateNames |
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| BackgroundInformation | H2A.Z,alsoknownasH2AFZ,isavariantofhistoneH2Avariantthatishighlyconservedfromyeasttohumans.Likeotherhistonevariants,H2A.Zisencodedbyauniquegene,resultinginaslightlydivergenttypeofH2Awithspecializedfunctions.H2A.ZisinvolvedinchromatincompactionbystABIlizingthehistoneoctamerwithinthenucleosome.Itpromotesgeneexpressionthroughthecreationofanopendomainofchromatinthatismoreeasilytranscribed.H2A.Zisnonrandomlydistributedthroughoutthegenome,targetedprimarilytopericentricheterochromatin,butexcludedfromtheinactiveXchromosomeandthenucleolus.H2A.Zisassociatedwithbotheuchromatinandfacultativeheterochromatin.DNAmethylationcaninfluencechromatinstructureandeffectgenesilencingbyexcludingH2A.Z,andlikewise,H2A.ZprotectsgenesfromDNAmethylation.Thus,H2A.Zplaysaroleinregulatingheterochromatinsilencingandisinvolvedintranscriptionalcontrol. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
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| Presentation | Purifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| Applications | |
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| KeyApplications |
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| ApplicationNotes | WesternBlot(Snapi.d.)Analysis:4µg/mLfromarepresentativelotdetectedHistoneH2A.Zon10µgofyeastwholecellextract. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedlinearpeptidecorrespondingtoyeastHistoneH2A.ZacetylatedatLys8. |
| Epitope | HistoneH2A.ZacetylatedLys8 |
| Concentration | 0.5mg/mL |
| Host | Rabbit |
| Specificity | ThisantibodyrecognizesHistoneH2A.ZwhenacetylatedatLys8. |
| SpeciesReactivity |
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| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:VarianthistoneH2AwhichcanreplaceH2Ainsomenucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Thisvariantisenrichedatpromoters,itmaykeeptheminarepressedstateuntiltheappropriateactivationsignalisreceived.Neartelomeres,itmaycounteractgenesilencingcausedbythespreadofheterochromatinproteins.RequiredfortheRNApolymeraseIIandSPT15/TBPrecruitmenttothetargetgenes.Involvedinchromosomestability. SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.H2AoritsvariantH2A.ZformsanheterodimerwithH2B.H2A.ZassociateswiththeVPS72/SWC2subunitoftheSWR1chromatinremodelingcomplex.InteractsalsowithRBP1/DNA-directedRNApolymeraseIIlargestsubunit.InteractswithNAP1. SUBCELLULARLOCATION:Nucleus PTM:AcetylatedbyESA1,acomponentoftheNuA4histoneacetyltransferase(HAT)complex,and/orbyGCN5,acomponentoftheSAGAcomplex,toformH2A.ZK3Ac,H2A.ZK8Ac,H2A.ZK10AcandH2A.ZK14Aconcedepositedintochromatin.Acetylationisrequiredforfunctionattelomeres.H2A.ZK14Acisacetylatedatthepromotersofactivegenes. MISCELLANEOUS:Presentwith2840molecules/cellinlogphaseSDmedium. IncontrasttoH2A1andH2A2,appearstobeweaklyornotsumoylated. SEQUENCESIMILARITIES:BelongstothehistoneH2Afamily. CAUTION:Toensureconsistencybetweenhistoneentries,wefollowthe"Brno"nomenclatureforhistonemodifications,withpositionsreferingtothoseusedintheliteratureforthe"closest"modelorganism.DuetoslightvariationsinhistonesequencesbetweenorganismsandtothepresenceofinitiatormethionineinUniProtKB/Swiss-Protsequences,theactualpositionsofmodifiedaminoacidsinthesequencegenerallydiffer.Inthisentrythefollowingconventionsareused:H2A.ZK3ac=acetylatedLys-4;H2A.ZK8ac=acetylatedLys-9;H2A.ZK10ac=acetylatedLys-11;H2A.ZK14ac=acetylatedLys-15. |
| MolecularWeight | ~16kDaobserved.Slightlycross-reactingband~14kDa(HistoneH4)withTBS-T(01%). |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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