Millipore/05-457 |抗组蛋白H1抗体，克隆AE-4/05-457/200µ；G
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 05-457 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-HistoneH1Antibody,cloneAE-4 |
| AlternateNames |
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| BackgroundInformation | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | PurifiedmousemonoclonalIgG2ainbuffercontainingPBS,pH7.2,with0.1%sodiumazide.Frozensolution. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations: Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | UseAnti-HistoneH1Antibody,cloneAE-4(mousemonoclonalantibody)validatedinELISA,ICC,WBtodetectHistoneH1alsoknownasH1histonefamilymember4,HistoneH1b,histone1H1e,histonecluster1H1e. |
| KeyApplications |
|
| ApplicationNotes | Immunocytochemistry: 5-20µg/mLofapreviouslotshowedpositivenuclearimmunostaininginA431cellsfixedwith4%paraformaldehydeandmethanol. ELISA: Recommended. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Nucleifrommyeloidleukemiabiopsycells.CloneAE-4. |
| Clone | AE-4 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | ThisantibodyrecognizeshistoneH1,Mr32-33kDa.DoubletdetectedduetohyperphosphorylationofhistoneH1subpopulation.Cross-reactswithotherhistoneisoformsinthefollowingorderofsignalstrength:H3,H2B>H4>H2A,asdeterminedwithrecombinantprotein. |
| Isotype | IgG2a |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Humanandbovine.Predictedtostainnucleifromotheranimalandplantspecies. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Histonesarebasicnuclearproteinsresponsiblefornucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH1family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6. |
| GeneSymbol |
|
| PurificationMethod | ProteinGPurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P10412#HistonesH1arenecessaryforthecondensationofnucleosomechainsintohigherorderstructures. SIZE:219aminoacids;21865Da SUBCELLULARLOCATION:Nucleus. SIMILARITY:SwissProt:P10412##BelongstothehistoneH1/H5family. MISCELLANEOUS:Thisvariantaccountsfor60%ofhistoneH1. |
| MolecularWeight | 32-33kDa(HistoneH1);Doublet(hyperphosphorylatedHistoneH1);17kDa(otherhistoneisoforms). |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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