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Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL
Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL
市场价：
¥5960.00
美元价：
3576.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3757 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-VEGFReceptor-3Antibody,extracellulardomain,clone9D9F9 |
| AlternateNames |
|
| BackgroundInformation | Vascularendothelialgrowthfactorreceptor-3(VEGFR-3)anditsligands,vascularendothelialgrowthfactor-C(VEGF-C)and-D(VEGF-D),arethemajormoleculesinvolvedindevelopmentalandpathologicallymphangiogenesis.{Bando,2004}.JussilaetalreportedthatVEGFR3antibodiesspecificallystainendothelialcellsoflymphaticvesselsandvesselsaroundtumonrssuchaslymphomaandinsitubreastcarcinoma.{Jussila,1998}.Interestingly,thespindlecellsofseveralcutaneousnodularAIDS-associatedKaposi"ssarcomasandtheendotheliumaroundthenoduleswerealsoVEGFR-3positive.ThefirstspecificmolecularMarkerforthelymphaticendotheliumshouldprovideausefultoolfortheanalysisoflymphaticvesselsinmalignanttumorsandtheirmetastasesandthecellularoriginanddifferentiationofKaposi"ssarcomas.{Jussila,1998}. |
| ProductInformation | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | UnPurifiedascitesfluidcontaining0.1%sodiumazideasapreservative. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | Anti-VEGFReceptor-3Antibody,extracellulardomain,clone9D9F9isanantibodyagainstVEGFReceptor-3foruseinFC,IF,IH(P),IP&WB. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry:frozencryosections,5μmcryosectionswereair-driedandfixedincoldacetonefor10minutes.Blockedwith5%normalhorseserumandthenincubatedwith9D9for2hoursatroomtemperatureinahumidchamber.Typicaldilutionswillrangefrom1:100-1:1000dependinguponlottiter.Detectioniswithenhancedmethods(ABCorpolyHRP)andenzymaticsubstratesforbestresults. Immunohistochemistryinparaffin:5μmthicklightlyfixedformalintissueswereprocessedthroughdecreasingethanolconcentrations,washedwithwater,thenheatedinamicrowavein10mMsodiumcitratepH6.0at780Wfor5minutes,followedby450Wfor10minutes.Thesectionswerethenincubatedinmethanolcontaining30%hydrogenperoxidefor30minutesandprocessedfurtherlikethecryosections.{Jussila,1998}. WesternBlot:antibodyrecognizesVEGFR-3onreducedsamplesofHUVECandHDMECcells{primayhumanumbilicalveinendothelialcells&primaryhumandermalmicrovascularendothelialcells}.Thebandsseenatmolecularweight195kDaand125kDarepresenttheuncleavedandproteolyticallycleavedformsofVEGFR3.Thebandat175kDaisconsideredtobeintracellular,unglycosylatedprecursor{Bando,2004}. Immunoprecipitation:1-5μLper300-500μLofcelllysate.UseproteinAorrabbitanti-mouseIgGcaptureforbestresults. ELISA:9D9F9hasbeenusedasthedetectionantibodyforaVEGFR-3ELISA{Bando,2004}. FlowCytometry:reactiveontransfectedNIH-3T3cellsexpressinghVEGFR3,Othercelllinesuntested.{Jussila,1998}. Optimaldilutionswillneedtobedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Recombinantlyproduced,purified,hVEGFR-3extracellulardomainproteinfrombaculovirusculture{Jussila,1998}. |
| Epitope | extracellulardomain |
| Clone | 9D9F9 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | SpecificforhumanVEGFR-3.WesternblotandELISAdemonstratethattheantibodyishighlyspecificforVEGFR-3withnocross-reactivitywithhumanVEGFR-1orVEGFR-2protein{Bando,2004}.CrossreactivitywithotherspeciesVEGFR-3proteinsuntested. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
|
| PurificationMethod | Unpurified |
| UniProtNumber | |
| MolecularWeight | 175kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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