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Millipore/MAB16985 | Anti-MCAM Antibody, clone P1H12/MAB16985/100 µg
Millipore/MAB16985 | Anti-MCAM Antibody, clone P1H12/MAB16985/100 µg
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB16985 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-MCAMAntibody,cloneP1H12 |
| AlternateNames |
|
| BackgroundInformation | MUC18antigen(CD146),amemberoftheimmuoglobulinsuperfamily,isanintrinsicmembraneglycoproteinof110-120kDafoundonthesurfaceofendothelialcells,bonemarrowfibroblastsandvariousmelanomas.MUC18hasbeenusedasaMarkeroftumorprogressioninhumanmelanomabecauseexpressioninthosetumorscorrelatesstronglywithpoorprognosisandthedevelopmentofmetasticdisease.Inaddition,anumberofhumanT,BandmyeloidleukemiccelllinesseemtoexpressMUC18.TheclosestructuralrelationshipwithN-CAMandrelatedmoleculessuggeststhatMUC18maybealsoadevelopmentallyregulatedcelladhesionmolecule(MelanomaadhesionmoleculeorMCAM). |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | PurifiedmousemonoclonalIgG1inbuffercontaining0.02MPBwith0.25MNaCl,pH=7.6,with0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Anti-MCAMAntibody,cloneP1H12detectslevelofMCAM&hasbeenpublished&validatedforuseinELISA,FC,IC,IH,IP&WB. |
| KeyApplications |
|
| ApplicationsNotRecommended |
|
| ApplicationNotes | Immunocytochemistry: 1-10μg/mLofapreviouslotworkedinimmunocytochemistry.WorksbestonEDTAorTrypsinliftedendothelialcells. Immunohistochemistry: 1-10µg/mL.4%PFAfor30minRTor<2hrs at="" 4°c.="" block="" w/="" 1%bsa/0.2%="" tween20/pbs="" for="" 30min.="" works="" well="" in="" frozen="" tissue;="" fixed="" or="" unfixed.=""> Immunoprecipitation: 1-10μg/mLofapreviouslotworkedinimmunoprecipitation. ELISA: 1-10μg/mLofapreviouslotworkedinELISA. FACSAnalysis: 1-10μg/mLofapreviouslotworkedinFACS. Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | ImmunizationofmicewithHUVEC. |
| Clone | P1H12 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | P1H12reactsspecificallywithCD146{MCAM,MUC18}.InbloodandmarrowP1H12reactsonlywithendothelialcells,andhasbeenusedtodetectcirculatingendothelialcellsinhuman,dogsandmousesamples.Itpositivelystainsnormal,primaryHUVECandMVECculturesandtheendothelialcellsofallvesselsinnormalfrozensectionsofhumanskin,intestine,ovarytonsil,lymphnode,lung,andkidney.ItdoesnotstaincarcinomacelllinesHT-29andCOLO205,andM21,theTcelllinesJurkatandHuT78,fibroblasts,HL-60orCHOcells,orEBV-transformedBcelllines,althoughitexpectedthatCD146expressionispresentinmanytumorlines.P1H12doesnotstainnormalmonocytes,granulocytes,redcells,platelets,TcellsorBcellsfrommarroworperipheralblood;nordoesitdetectmarrowmegakaryocytesorthemegakaryoblastlineHU3.TheperipheralbloodcellsthatdostainwithP1H12alsoarepositiveforbothvonWillebrandFactor(vWF)andthrombomodulin(thecombinedexpressionofwhichislimitedtoendothelium),andtheystainforfltandflk.Inrecenttesting,melanomaA2058,SKMEL.3andA375.S2arepositive,whilemurinemelanomaM3isnegative.Onesmoothmuscleline(HISM)waspositiveandonenegative(TIGHA-VSMC).Humanbloodlymphoctesarenegativewithorwithoutpriorstimulation. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Detectscirculatingendothelialcellsinhuman.Expectedtocross-reactwithdog&mouse. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
|
| Non-ReactiveSpecies |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P43121#Playsaroleincelladhesion,andincohesionoftheendothelialmonolayeratintercellularjunctionsinvasculartissue.Itsexpressionmayallowmelanomacellstointeractwithcellularelementsofthevascularsystem,therebyenhancinghematogeneoustumorspread.Couldbeanadhesionmoleculeactiveinneuralcrestcellsduringembryonicdevelopment.ActsassurfacereceptorthattriggerstyrosinephosphorylationofFYNandPTK2,andatransientincreaseintheintracellularcalciumconcentration. SIZE:646aminoacids;71607Da SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Detectedinendothelialcellsinvasculartissuethroughoutthebody.Mayappearatthesurfaceofneuralcrestcellsduringtheirembryonicmigration.Appearstobelimitedtovascularsmoothmuscleinnormaladulttissues.Associatedwithtumorprogressionandthedevelopmentofmetastasisinhumanmalignantmelanoma.ExpressedmoststronglyonmetastaticlesionsandadvancedprimarytumorsandisonlyrarelydetectedinbenignmelanocyticneviandthinprimarymelanomaswithalowprobABIlityofmetastasis. SIMILARITY:SwissProt:P43121##Contains3Ig-likeC2-type(immunoglobulin-like)domains.&Contains2Ig-likeV-type(immunoglobulin-like)domains. |
| MolecularWeight | 110kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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