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Millipore/09-310 | Anti-phospho-EGFR (Tyr1069) Antibody/09-310/100 µL
Millipore/09-310 | Anti-phospho-EGFR (Tyr1069) Antibody/09-310/100 µL
市场价：
¥5540.00
美元价：
3324.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 09-310 |
| Replaces | 04-339 |
| Description | Anti-phospho-EGFR(Tyr1069)Antibody |
| AlternateNames |
|
| BackgroundInformation | EGFR(Epidermalgrowthfactorreceptor,ErbB-1)isareceptortyrosinekinase(RTK)thatisoneoffourmembersoftheEGFR/ErbBfamilyofreceptortyrosinekinases.EGFRplaysakeyroleintheregulationofessentialnormalcellularprocessesandinthepathophysiologyofhyperproliferativediseasessuchascancer.ActivationoftheEGFRsignalingpathwayhasbeenlinkedwithincreasedcellproliferation,angiogenesis,metastasisanddecreasedapoptosis.Uponligandbinding,EGFRundergoesatransitionfromaninactivemonomericformtoanactivedimer.Inadditiontoforminghomodimers,EGFRmaypairwithanothermemberoftheErbBreceptorfamily,suchasErbB2/Her2/neu,tocreateanactivatedheterodimer.EGFRdimerizationstimulatesitsintrinsicintracellularprotein-tyrosinekinaseactivitythatleadstotrans-andauto-phosphorylationoftyrosineresidueswithinthecytoplasmicdomainofthereceptortyrosinekinaseoffivetyrosine(Y)residuesonsitesY992,Y1045,Y1068,Y1148andY1173locatedontheC-terminaldomainofEGFR.Thisautophosphorylationelicitsdownstreamactivationofseveralsignaltransductioncascades,principallytheMAPK,AktandJNKpathwaysthroughRas,PI3Kinase,andPLCpathwaysthatultimatelyleadstotheactivationofthevariouspathwaysdownstream.Tyr1069isphosphorylatedduringactivation. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
|
| Presentation | Antigenaffinitypurifiedrabbitpolyclonalin0.1MTris-Glycine(pH7.4)150mMNaCl,with0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8ºCinundilutedaliquotsfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution. |
| Applications | |
|---|---|
| Application | ThisAnti-phospho-EGFR(Tyr1069)AntibodyisvalidatedforuseinELISA,IC,WBforthedetectionofphospho-EGFR(Tyr1069). |
| KeyApplications |
|
| ApplicationNotes | ImmunofluroescentImmunocytochemistry: |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Tyrosine-phosphorylatedepidermalgrowthfactorreceptor. |
| Epitope | Tyr1069(cytoplasmicdomain) |
| Host | Rabbit |
| Specificity | ReactsstronglyandspecificallywithTyr1069phosphorylatedEGFR |
| SpeciesReactivity |
|
| SpeciesReactivityNote | TestedinHuman.Expectedtoreactwithmouse,rat,primate,horseandpigbasedon100%sequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Theproteinencodedbythisgeneisatransmembraneglycoproteinthatisamemberoftheproteinkinasesuperfamily.Thisproteinisareceptorformembersoftheepidermalgrowthfactorfamily.EGFRisacellsurfaceproteinthatbindstoepidermalgrowthfactor.Bindingoftheproteintoaligandinducesreceptordimerizationandtyrosineautophosphorylationandleadstocellproliferation.Mutationsinthisgeneareassociatedwithlungcancer.[providedbyRefSeq] |
| GeneSymbol |
|
| Modifications |
|
| PurificationMethod | AntigenAffinityPurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:ReceptorforEGF,butalsoforothermembersoftheEGFfamily,asTGF-alpha,amphiregulin,betacellulin,heparin-bindingEGF-likegrowthfactor,GP30andvacciniavirusgrowthfactor.Isinvolvedinthecontrolofcellgrowthanddifferentiation.PhosphorylatesMUC1inbreastcancercellsandincreasestheinteractionofMUC1withC-SRCandCTNNB1/beta-catenin.Ref.32 Isoform2/truncatedisoformmayactasanantagoNIST.Ref.32 CatalyticactivityATP+a[protein]-L-tyrosine=ADP+a[protein]-L-tyrosinephosphate. SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1.BindsMUC1.Ref.32Ref.26Ref.30Ref.31Ref.36 SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein. Isoform2:Secreted. TISSUESPECIFICITY:Ubiquitouslyexpressed.Isoform2isalsoexpressedinovariancancers.Ref.39 PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.Ref.17Ref.25Ref.29Ref.33Ref.34Ref.35Ref.37Ref.38Ref.40Ref.41Ref.42Ref.43 MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur. DISEASE:DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. MiscellaneousBindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation. SIMILARITY:Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily. Contains1proteinkinasedomain. |
| MolecularWeight | ~170kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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