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Millipore/07-1024 | Anti-EGFR Antibody, cytoplasmic domain/07-1024/100 µg
Millipore/07-1024 | Anti-EGFR Antibody, cytoplasmic domain/07-1024/100 µg
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产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-1024 |
| Description | Anti-EGFRAntibody,cytoplasmicdomain |
| AlternateNames |
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| BackgroundInformation | EGFR(EpidermalGrowthFactorReceptor;ErbB-1;HER1)isthecell-surfacereceptorformembersoftheepidermalgrowthfactorfamily(EGF-family)ofextracellularproteinligands.TheEGFreceptorisamemberoftheErbBfamilyofreceptors,asubfamilyoffourcloselyrelatedreceptortyrosinekinases:EGFR(ErbB-1),HER2/c-neu(ErbB-2),Her3(ErbB-3)andHer4(ErbB-4).MutationsaffectingEGFRexpressionoractivitycouldresultincancer. EGFRdimerizationstimulatesitsintrinsicintracellularprotein-tyrosinekinaseactivity.Asaresult,autophosphorylationoffivetyrosine(Y)residues(Y992,Y1045,Y1068,Y1148andY1173)intheC-terminaldomainofEGFRoccurs.Thisautophosphorylationelicitsdownstreamactivationandsignalingbyseveralotherproteinsthatassociatewiththephosphorylatedtyrosinesthroughtheirownphosphotyrosine-bindingSH2domains. Thesedownstreamsignalingproteinsinitiateseveralsignaltransductioncascades,principallytheMAPK,AktandJNKpathways,leADIngtoDNAsynthesisandcellproliferation. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Presentation | Affinitypurifiedrabbitpolyclonalin0.1MTris-Glycine(pH7.4)with150mMNaCland0.05%NaN3. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution. |
| Applications | |
|---|---|
| Application | ThisAnti-EGFRAntibody,cytoplasmicdomainisvalidatedforuseinWBforthedetectionofEGFR. |
| KeyApplications |
|
| BIOLOGicalInformation | |
|---|---|
| Immunogen | GSTfusionproteincorrespondingtothecytoplasmicdomainofhumanEGFR. |
| Epitope | CytoplasmicDomain |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | DetectsEGFR |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.Otherspecieshavenotbeentested. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Theproteinencodedbythisgeneisatransmembraneglycoproteinthatisamemberoftheproteinkinasesuperfamily.Thisproteinisareceptorformembersoftheepidermalgrowthfactorfamily.EGFRisacellsurfaceproteinthatbindstoepidermalgrowthfactor.Bindingoftheproteintoaligandinducesreceptordimerizationandtyrosineautophosphorylationandleadstocellproliferation.Mutationsinthisgeneareassociatedwithlungcancer.[providedbyRefSeq] |
| GeneSymbol |
|
| PurificationMethod | ImmunogenAffinityPurified |
| UniProtNumber | |
| UniProtSummary | Function:ReceptorforEGF,butalsoforothermembersoftheEGFfamily,asTGF-alpha,amphiregulin,betacellulin,heparin-bindingEGF-likegrowthfactor,GP30andvacciniavirusgrowthfactor.Isinvolvedinthecontrolofcellgrowthanddifferentiation.PhosphorylatesMUC1inbreastcancercellsandincreasestheinteractionofMUC1withC-SRCandCTNNB1/beta-catenin.Ref.31 Isoform:2/truncatedisoformmayactasanantagoNIST.Ref.31 Catalyticactivity:ATP+a[protein]-L-tyrosine=ADP+a[protein]-L-tyrosinephosphate. Subunitstructure:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1.BindsMUC1.Ref.31Ref.25Ref.29Ref.30Ref.35 Subcellularlocation:Cellmembrane;Single-passtypeImembraneprotein. Isoform:2:Secreted. Tissuespecificity:Ubiquitouslyexpressed.Isoform2isalsoexpressedinovariancancers.Ref.38 Post-translationalmodification:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.Ref.16Ref.24Ref.28Ref.32Ref.33Ref.34Ref.36Ref.37Ref.39Ref.40Ref.41Ref.42 MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur. Involvementindisease:DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. Miscellaneous:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation. Sequencesimilarities:Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily. Contains1proteinkinasedomain. |
| MolecularWeight | 180kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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