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当前位置: 首页 > 产品中心 > Functional_antibody > 微孔/AB1599 |抗磷酸酪氨酸抗体/AB1599/100µ;G
商品详细微孔/AB1599 |抗磷酸酪氨酸抗体/AB1599/100µ;G
微孔/AB1599 |抗磷酸酪氨酸抗体/AB1599/100µ;G
微孔/AB1599 |抗磷酸酪氨酸抗体/AB1599/100µ;G
商品编号: AB1599
品牌: 密理博
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
Catalogue NumberAB1599
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Phosphotyrosine Antibody
Product Information
FormatAffinity Purified
Control
  • POSITIVE: EGF-stimulated A431 cells.
PresentationAffinity purified immunoglobulin in 0.05m Tris with 0.15M NaCl, pH=7.2, and 0.02% sodium azide.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -20°C in undiluted aliquots. For working stocks, it is suggested to dilute the stock 20μL with a solution of 1% BSA in TBS (pH 7.4, that has been filtered through a 0.45μm membrane filter) to enhance the full recovery of the antibody from the supplied tube. Avoid repeated freeze/thaw.
Applications
ApplicationDetect Phosphotyrosine using this Anti-Phosphotyrosine Antibody validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1:5,000 - 1:30,000 using colorimetric detection. Detection with more sensitive systems, such as chemiluminescence, may require higher antibody dilution. At a dilution of 1:1,000 the antibody was easily able to detect 10 ng (0.2 pmol) of a phosphorylated peptide using chromogenic (BCIP/NBT) development.

Slot blot: 1:10,000.

ELISA: 1:30,000 to 1;100,000 for detection of 1 μg/well of phosphotyrosine-containing substrate.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenA random amino acid polymer containing phosphotyrosine which was conjugated to Keyhole Limpet Hemocyanin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificitySpecific for phosphotyrosine. No reaction with phosphoserine, phosphothreonine, serine, threonine, or tyrosine as confirmed by ELISA and Western blot using phosphorylated and unphosphosphorylated proteins.
Species Reactivity
  • All
Antibody TypePolyclonal Antibody
Modifications
  • Phosphorylation
Physicochemical Information
Dimensions
Materials Information
Materials Information
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。