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Millipore/16-105 | Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate/16-105/10 blots
Millipore/16-105 | Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate/16-105/10 blots
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产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 16-105 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-PhosphotyrosineAntibody,clone4G10®,HRPconjugate |
| Overview | Anti-Phosphotyrosineantibody(05-321),cross-linkedtohorserADIshperoxidase |
| BackgroundInformation | Someofthetyrosineresiduescanbetaggedwithaphosphategroup(phosphorylated)byproteinkinases.(Initsphosphorylatedstate,itisreferredtoasphosphotyrosine).Tyrosinephosphorylationisconsideredasoneofthekeystepsinsignaltransductionandregulationofenzymaticactivity.Theadventofanti-phospho-tyrosineantibodiesisoneofsignificanteventsinsignaltransductionresearch.BeforetheavailABIlityofanti-phosphotyrosineantibodies,tyrosylphospyhorylationofproteinsandenzymeswasinvestigatedthroughhazardousandtime-consumingradioactiveexperiments.Anti-phosphotyrosineantibodiesarecommonlyusedinwesternblotsafterthetargetedproteinshavebeenimmunoprecipitatedtomeasurethetyrosylphosphorylationoftheproteins.Anti-phosphotyrosineantibodiesarealsodirectlyusedoncelllysatetoexaminetheoverallchangeoftyrosinephosphorylationlevelinresponsetovarioustreatments. Description IgG2bκmousemonoclonalantibodyproducedinvitrobymouse-mousehybridoma4G10®.PurifiedbyProteinG-Sepharosechromatographyandcross-linkedtohorseradishperoxidase. |
| ProductInformation | |
|---|---|
| Format | HRP |
| Control |
|
| Presentation | 200μgofIgG2bκ-HRPconjugatein100μLPBScontaining0.05%Kathon®.Sufficientfor10westernblots,ifusedattheoptimumconcentrationof2μg/mLin10mLblockingbuffer.Liquid. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor6monthsat2-8°Cfromdateofreceipt.NOTE:DONOTFREEZE.Formaximumrecoveryofproduct,centrifugetheoriginalvialpriortoremovingthecap. NOTE:Foroptimalperformance,removeHRPprecipitatebycentrifugingvialpriortouse. |
| Applications | |
|---|---|
| Application | Anti-PhosphotyrosineAntibody,clone4G10®,HRPconjugatedetectslevelofPhosphotyrosine&hasbeenpublished&validatedforuseinELISA&WB. |
| KeyApplications |
|
| ApplicationNotes | ForuseinapplicationsinwhichanHRPconjugateisadvantageous,suchasWBandIP. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Phosphotyramine-KLH. |
| Epitope | N/A |
| Clone | 4G10® |
| Host | Mouse |
| Specificity | Recognizestyrosine-phosphorylatedproteinsfromallspecies. |
| Isotype | IgG2bκ |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| Modifications |
|
| PurificationMethod | ProteinG-Sepharose™Chromatography |
| MolecularWeight | Dependentuponthemolecularweightofthetyrosinephosphorylatedproteinbeingdetected. |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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