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Millipore/06-427 | Anti-Phosphotyrosine Antibody/06-427/100 µL
Millipore/06-427 | Anti-Phosphotyrosine Antibody/06-427/100 µL
市场价：
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美元价：
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产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 06-427 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-PhosphotyrosineAntibody |
| BackgroundInformation | Thephosphorylationofspecifictyrosineresidueshasbeenshowntobeaprimarymechanismofsignaltransductionduringnormalmitogenesis,cellcycleprogressionandoncogenictransformation,itsroleinotherareassuchasdifferentiationandgapjunctioncommunication,isamatterofactiveandongoingresearch.Antibodiesthatspecificallyrecognizephosphorylatedtyrosineresidueshaveprovedtobeinvaluabletothestudyoftyrosinephosphorylatedproteinsandthebiochemicalpathwaysinwhichtheyfunction. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Presentation | Purifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Anti-PhosphotyrosineAntibodydetectslevelofPhosphotyrosine&hasbeenpublished&validatedforuseinIP&WB. |
| KeyApplications |
|
| ApplicationNotes | WesternBlottingAnalysis:A1:500dilutionfromarepresentativelotdetectedPhosphotyrosinein10µgofEGFtreatedA431withLamBDaPhosphatasetissuelysate. ImmunoprecipitationAnalysis:5µLfromarepresentativelotimmunoprecipitatedPhosphotyrosinein0.5mgofEGFtreatedA431celllysate |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Inordertoproducebroadspectrumpolyclonalphosphotyrosineantibodies,rabbitswereimmunizedwiththreephosphorylatedimmunogens:(1)phosphotyrosinecovalentlylinkedtoKLH;(2)thec-Srccarboxylterminalregulatoryphosphopeptide(TS-T-E-P-Q-pY-Q-P-G-E-N-L;Catalog#12-218)covalentlylinkedtoKLH,and;(3)aphosphopeptideassociatedwithhightyrosinekinaseactivityinhumanlymphocytes(R-R-L-I-E-D-A-E-pY-A-A-R-G;Catalog#12-217)covalentlylinkedtoKLH.BothofthephosphopeptidehaptensserveasstrongsubstratesfortyrosinephosphatasesandarepartofthetwocolorimetrictyrosinephosphatasekitsprovidedbyUpstate,Inc.(Catalog#17-125,17-126). |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | Theimmunoreactivityoftheantibodyistotallyinhibitedbytheuseof100mMphenylphosphate,aphosphotyrosineanalog.Thephosphotyrosineantibodyispurifiedbyimmunoaffinitychromatographyusingeitheradualphospho-peptidegeloraBSA-phosphotyrosinegel.Allofthephosphotyrosineimmunoreactivitypresentintheantiseraisimmunoadsorbedwhethertheantibodyispurifiedbyeithergelindicatingthattheantibodyisnotsequence-specificbutspecificforphosphotyrosineresidues. |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.PredictedtoreactwithAllbasedon100%sequencehomology. |
| AntibodyType | PolyclonalAntibody |
| Modifications |
|
| PurificationMethod | AffinityPurfied |
| MolecularWeight | Varies |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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