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当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/05-321X |抗磷酸酪氨酸抗体,克隆4G10®/05-321X/50µ;G
商品详细Millipore/05-321X |抗磷酸酪氨酸抗体,克隆4G10®/05-321X/50µ;G
Millipore/05-321X |抗磷酸酪氨酸抗体,克隆4G10®/05-321X/50µ;G
Millipore/05-321X |抗磷酸酪氨酸抗体,克隆4G10®/05-321X/50µ;G
商品编号: 05-321X
品牌: 密理博
市场价: ¥5120.00
美元价: 3072.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumber05-321X
BrandFamilyUpstate
TradeName
  • Upstate
DescriptionAnti-PhosphotyrosineAntibody,clone4G10®
BackgroundInformationSomeofthetyrosineresiduescanbetaggedwithaphosphategroup(phosphorylated)byproteinkinases.(Initsphosphorylatedstate,itisreferredtoasphosphotyrosine.).Tyrosinephosphorylationisconsideredasoneofthekeystepsinsignaltransductionandregulationofenzymaticactivity.
Theadventofanti-phosphotyrosineantibodiesisoneofsignificanteventsinsignaltransductionresearch.BeforetheavailABIlityofanti-phosphotyrosineantibodies,tyrosylphospyhorylationofproteinsandenzymeswasinvestigatedthroughhazardousandtime-consumingrADIoactiveexperiments.Anti-phosphotyrosineantibodiesarecommonlyusedinwesternblotsafterthetargetedproteinshavebeenimmunoprecipitatedtomeasurethetyrosylphosphorylationoftheproteins.Anti-phosphotyrosineantibodiesarealsodirectlyusedoncelllysatetoexaminetheoverallchangeoftyrosinephosphorylationlevelinreponsetovarioustreatments.
ProductInformation
FormatPurified
Control
  • UntreatedA-431(negativecontrol)andEGFtreatedA-431(positivecontrol)wholecelllysates.
    IncludedPositiveAntigenControl:Catalog#12-302,EGF-stimulatedA431celllysateisprovidedasafreepositiveantigencontrolforwesternimmunoblotting.Aliquotasdesired,refreezeimmediately,andstoreat-20°C.Thelysateisstablefor6monthsat-20°C.Beforeuse,add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforimmunoblotanalysis.
PresentationPurifiedmousemonoclonalIgG2bκinbuffercontaining0.1MTris-glycine,pH7.4,0.15MNaCl,0.05%sodiumazide.Liquidat2-8ºC.
IgG2bκmousemonoclonalantibodyproducedinvitrobymouse-mousehybridoma4G10®(FOX-NY[NS-1derivative]myelomaxspleencells).
StorageandShippingInformation
StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.

NOTE:DONOTFREEZE.
Formaximumrecoveryoftheproduct,centrifugetheoriginalvialpriortoremovingthecap.Iftheproducthasaccidentallybeenfrozenandthawed,spinitat13,000xgfor10minutesat2-8ºC.Savethesupernatantforapplication.
Applications
ApplicationAnti-PhosphotyrosineAntibody,clone4G10detectstyrosinephosphorylatedproteinsinallspecies.ThisuniquemonoclonalantibodyisvalidatedforuseinIC,IH,IP,WBandisbackedbyhundredsofpublications.
KeyApplications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • WesternBlotting
ApplicationNotesImmunoprecipitation:
2-4μgofapreviouslotimmunoprecipitatedquantitativelythephosphotyrosinecontainingproteinsinthelysateofaconfluentculture(10cmdish)ofcellsexpressinganactivatedtyrosinekinase.Topreservephosphotyrosine,add:0.2mMsodiumorthovanadatetothelysisbuffer.
BIOLOGicalInformation
ImmunogenPhosphotyramine-KLH
Clone4G10®
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostMouse
SpecificityThisantibodyrecognizestyrosine-phosphorylatedproteinsfromallspecies.
IsotypeIgG2bκ
SpeciesReactivity
  • Vertebrates
AntibodyTypeMonoclonalAntibody
Modifications
  • Phosphorylation
PurificationMethodProteinG-Sepharose™Chromatography
MolecularWeightDependentuponthemolecularweightofthetyrosinephosphorylatedproteinbeingdetected.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。