4000-520-616
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当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/05-1050 | 4G10®铂,抗磷酸酪氨酸抗体(小鼠单克隆鸡尾酒IgG2b)/05-1050/100µ;L
商品详细Millipore/05-1050 | 4G10®铂,抗磷酸酪氨酸抗体(小鼠单克隆鸡尾酒IgG2b)/05-1050/100µ;L
Millipore/05-1050 | 4G10®铂,抗磷酸酪氨酸抗体(小鼠单克隆鸡尾酒IgG2b)/05-1050/100µ;L
Millipore/05-1050 | 4G10®铂,抗磷酸酪氨酸抗体(小鼠单克隆鸡尾酒IgG2b)/05-1050/100µ;L
商品编号: 05-1050
品牌: 密理博
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumber05-1050
BrandFamilyUpstate
TradeName
  • Upstate
Description4G10®Platinum,Anti-PhosphotyrosineAntibody(mousemonoclonalcocktailIgG2b)
BackgroundInformationThedevelopmentoftheanti-phosphotyrosine,clone4G10®in1989wasamonumentaldiscoveryforresearchers.4G10®wasthefirstandisthebestsinglemonoclonalantibodyforthedetectiontyrosinephosphorylation.4G10®iswellknownforitssensitivityanditsABIlitytodetectmultipletyrosinephosphorylationsonnumeroussubstrates.Ithasbeenvalidatedbythousandsofscientificandmedicalresearchersinvirtuallyeveryapplicationandtyrosinetargetoverthepast2decades.Toimproveonsomethingthathundredhavetriedandnoonehassucceeded,wepooled4G10®withthenextmosthighlyregardedanti-phosphotyrosine,clonePY20tomake4G10®Platinum.PY20itselfisaverypoorsubstitutefor4G10®,butitsadditiveeffectallowforagreaterlevelofdetectiononmoresubstratesthateven4G10®alonewasnotcapableof.
ProductInformation
FormatPurified
Control
  • SuggestedPositiveAntigenControl:Cat.#12-302,EGF-stimulatedA431celllysateisprovidedasafreepositiveantigencontrolforwesternimmunoblotting.Aliquotasdesired,refreezeimmediately,andstoreat-20ºC.Thelysateisstablefor6monthsat-20ºC.Beforeuse,add2.5μLof2-mercaptoethanolper100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforimmunoblotanalysis.
PresentationProprietarymixtureofproteinGpurifiedmousemonoclonals4G10®(IgG2b)andPY20(IgG2b)in0.1MTris-glycine,Ph7.4,0.15MNaCl,0.05%sodiumazide.
Liquidat2-8°C.
StorageandShippingInformation
StorageConditionsStablefor1yearatfromdateofreceipt.
Applications
Application4G10Platinum,Anti-Phosphotyrosine(mousemonoclonalcocktailIgG2b)detectslevelsofPhosphotyrosineproteins&hasbeenpublished&validatedforuseinWB,ICC,ELISA,IHC,IF,Flow&IP.
KeyApplications
  • WesternBlotting
  • Immunocytochemistry
  • ELISA
  • Immunohistochemistry
  • Immunofluorescence
  • FlowCytometry
  • Immunoprecipitation
ApplicationNotesImmunoprecipitation:2-4μLofapreviouslotcanimmunoprecipitatequantitativelythephosphotyrosinecontainingproteinsinthelysateofaconfluentculture(10cmdish)ofcellsexpressinganactivatedtyrosinekinase.Topreservephosphotyrosine,add0.2mMsodiumorthovanadatetothelysisbuffer.
BIOLOGicalInformation
Immunogen4G10®usedPhosphotyraminecoupledtoKLHandPY20usedphosphotyrosineconjugatedtocarrierprotein
Clone4G10®
HostMouse
SpecificityTyrosine-phosphorylatedproteinsfromallspecies
IsotypeIgG2b
SpeciesReactivity
  • All
AntibodyTypeMonoclonalAntibody
Modifications
  • Phosphorylation
PurificationMethodProteinG-Sepharose™Chromatography
MolecularWeightDependentuponthemolecularweightofthetyrosinephosphorylatedproteinbeingdetected.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。