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Millipore/MAB386 | Anti-Myelin Basic Protein Antibody, a.a. 82-87/MAB386/1 mL
Millipore/MAB386 | Anti-Myelin Basic Protein Antibody, a.a. 82-87/MAB386/1 mL
市场价：
¥6160.00
美元价：
3696.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB386 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-MyelinBasicProteinAntibody,a.a.82-87 |
| AlternateNames |
|
| BackgroundInformation | ThetwomajorcomponentsofCNSmyelinareMyelinBasicProtein(MBP)andproteolipidprotein(PLP),occuringinroughlyequimolarproportions.Therearemanyneurologicaldisordersassociatedwithdeficienciesofmyelinassemblyandstructure.ForexampleMultipleSclerosisisahumandiseaseduetomultiplefactorsbutgenerallycharacterizedbythedegradationofthemyelinsheath.MBPususallyreferstothe18.5kDaisoform,whichisoneofthemostabundantproteinsofthemyelinsheathofadulthumanandbovineCNS.Genetically,MBPistheproductofdifferentialsplicingofasinglemRNAtranscript.Inhumansthereare4isoforms,21.5,20.2,18.5,and17.2kDa.Thus,MBParepresentinmultipleisoforms,partofalargefamilyofdevelopmentallyexpressed,translocatable,andhighlypost-translationallymodifedproteinswithmultiplebindingpartners.Structurally,MBPdisplaysapredominatelyunorganizedformwithgreaterthan75%inrandomcoil.Othernon-CNSrelatedfunctionsofmyelinincludeinsulininductionandglucagonreleasefrompancreaticislets. |
| ProductInformation | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Liquidcontaining0.09%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Anti-MyelinBasicProteinAntibody,a.a.82-87isanantibodyagainstMyelinBasicProteinforuseinELISA,IC,IH,RIA&WB. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry: Apreviouslotofthisantibodywasusedonfrozensections. ELISA/RIA: 1:1000dilutionofapreviouslotofthisantibodywasusedinELISA/RIA. WesternBlotting: Identifiesan18-20kDabandinwesterns. Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Bovinemyelinbasicprotein |
| Epitope | a.a.82-87 |
| Clone | 12 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rat |
| Specificity | ReactswithMBPfromallspeciestested.Itreactsweaklywithpeptidesendinginthephe91wherethe91-92phe-phebondisbroken.Syntheticpeptide82-99reactsverywellasdoesintactMBP.MappedtotheregionDENPVV. |
| Isotype | IgG2a |
| SpeciesReactivity |
|
| SpeciesReactivityNote | ReactswithMBPfromallspeciestestedincludinghuman,bovine,sheep,rabbit,mouse,rat,guineapigandchicken. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbytheclassicMBPgeneisamajorconstituentofthemyelinsheathofoligodendrocytesandSchwanncellsinthenervoussystem.However,MBP-relatedtranscriptsarealsopresentinthebonemarrowandtheimmunesystem.ThesemRNAsarisefromthelongMBPgene(otherwisecalled"Golli-MBP")thatcontains3additionalexonslocatedupstreamoftheclassicMBPexons.AlternativesplicingfromtheGolliandtheMBPtranscriptionstartsitesgivesriseto2setsofMBP-relatedtranscriptsandgeneproducts.TheGollimRNAscontain3exonsuniquetoGolli-MBP,splicedin-frameto1ormoreMBPexons.TheyencodehybridproteinsthathaveN-terminalGolliaasequencelinkedtoMBPaasequence.ThesecondfamilyoftranscriptscontainonlyMBPexonsandproducethewellcharacterizedmyelinbasicproteins.ThiscomplexgenestructureisconservedamongspeciessuggestingthattheMBPtranscriptionunitisanintegralpartoftheGollitranscriptionunitandthatthisarrangementisimportantforthefunctionand/orregulationofthesegenes |
| GeneSymbol |
|
| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P02686#TheclassicgroupofMBPisoforms(isoform4-isoform14)arewithPLPthemostabundantproteincomponentsofthemyelinmembraneintheCNS.TheyhavearoleinbothitsformationandstABIlization.Thesmallerisoformsmighthaveanimportantroleinremyelinationofdenudedaxonsinmultiplesclerosis.Thenon-classicgroupofMBPisoforms(isoform1-isoform3/Golli-MBPs)maypreferentiallyhavearoleintheearlydevelopingbrainlongbeforemyelination,maybeascomponentsoftranscriptionalcomplexes,andmayalsobeinvolvedinsignalingpathwaysinT-cellsandneuralcells.Differentialsplicingeventscombinedtooptionalpost-translationalmodificationsgiveawidespectrumofisomers,eachofthemhavingmaybeaspecializedfunction.InducesT-cellproliferation. SIZE:304aminoacids;33117Da SUBUNIT:Homodimer;isoform3existsasahomodimer. SUBCELLULARLOCATION:Myelinmembrane;Peripheralmembraneprotein;Cytoplasmicside.Note=Cytoplasmicsideofmyelin. TISSUESPECIFICITY:MBPisoformsarefoundinboththecentralandtheperipheralnervoussystem,whereasGolli-MBPisoformsareexpressedinfetalthymus,spleenandspinalcord,aswellasincelllinesderivedfromtheimmunesystem. DEVELOPMENTALSTAGE:Expressionturnsonabruptlyinfetusof14to16weeks.Evensmallerisoformsseemtobeproducedduringembryogenesis,someofthesepersistingintheadult.ExpressionofisoformMBP2ismoreevidentat16weeksanditsrelativeproportiondeclinedthereafter. PTM:SeveralchargeisomersofMBP;C1(themostcationic,leastmodified,andmostabundantform),C2,C3,C4,C5,C6,C7,C8-AandC8-B(theleastcationicform);areproducedasaresultofoptionalPTM,suchasphosphorylation,deamidationofglutamineorasparagine,argininecitrullinationandmethylation.C8-AandC8-BcontaineachtwomassisoformstermedC8-A(H),C8-A(L),C8-B(H)andC8-B(L),(H)standingforhigherand(L)forlowermolecularweight.C3,C4andC5arephosphorylated.Theratioofmethylatedarginineresiduesdecreasesinaging,makingtheproteinmorecationic.&TheN-terminalalanineisacetylated(isoform3,isoform4,isoform5andisoform6).&Arg-241wasfoundtobe6%monomethylatedand60%symmetricallydimethylated. DISEASE:SwissProt:P02686#Thereductioninthesurfacechargeofcitrullinatedand/ormethylatedMBPcouldresultinaweakenedattachmenttothemyelinmembrane.Thismechanismcouldbeoperativeindemyelinatingdiseasessuchaschronicalmultiplesclerosis(MS),andfulminatingMS(Marburgdisease). SIMILARITY:SwissProt:P02686##Belongstothemyelinbasicproteinfamily. |
| MolecularWeight | 18-20kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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