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Millipore/MAB382 | Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1/MAB382/2 mL
Millipore/MAB382 | Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1/MAB382/2 mL
市场价：
¥9040.00
美元价：
5424.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB382 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-MyelinBasicProteinAntibody,a.a.129-138,clone1 |
| AlternateNames |
|
| BackgroundInformation | TheclassicgroupofMBPisoforms(isoforms4-14)arewithPLPthemostabundantproteincomponentsofthemyelinmembraneintheCNS.TheyhavearoleinbothitsformationandstABIlization.Thesmallerisoformsmighthaveanimportantroleinremyelinationofdenudedaxonsinmultiplesclerosis.Thenon-classicgroupofMBPisoforms(isoforms1-3/Golli-MBPs)maypreferentiallyhavearoleintheearlydevelopingbrainlongbeforemyelination,maybeascomponentsoftranscriptionalcomplexes,andmayalsobeinvolvedinsignalingpathwaysinT-cellsandneuralcells.Differentialsplicingeventscombinedtooptionalposttranslationalmodificationsgiveawidespectrumofisomers,eachofthemhavingmaybeaspecializedfunction. MBPisoformsarefoundinboththecentralandtheperipheralnervoussystem,whereasGolli-MBPisoformsareexpressedinfetalthymus,spleenandspinalcord,aswellasincelllinesderivedfromtheimmunesystem. Isoform1Golli-MBP1,HOG7,33kDa Isoform2Golli-MBP2,HOG5,21.5kDa Isoform3MBP1,21.5kDa Isoform4MBP2,20.2kDa Isoform5MBP3,18.5kDa Isoform6MBP4,17.2kDa (SP_P02686) |
| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Control |
|
| Presentation | Culturesupernatantcontaining0.2MTris/HCl,pH7.4with5-10%fetalcalfserumand0.1%sodiumazide |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCinundilutedaliquotsfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | ThisAnti-MyelinBasicProteinAntibody,a.a.129-138,clone1isvalidatedforuseinELISA,IH,IH(P),RIA,WBforthedetectionofMyelinBasicProtein. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry(paraffin)Analysis: OptimalStainingWithCitrateBuffer,pH6.0,EpitopeRetrieval:RatCerebellum Immunohistologyonfrozensectionsat1:10 WesternBlotAnalysis: ApreviouslotofthisantibodywasusedinWesternBlot. ELISA: A1:200-1:1,000dilutionofapreviouslotwasusedinELISA. RIA: ApreviouslotofthisantibodywasusedinRadioimmunoassay. Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Bovinemyelinbasicprotein |
| Epitope | a.a.129-138 |
| Clone | 1 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | ReactswithMBPfromhuman,bovineandrat,epitope129-138 |
| Isotype | IgG2a |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Reactsweaklywithrabbit.Doesnotreactwithguineapig. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbytheclassicMBPgeneisamajorconstituentofthemyelinsheathofoligodendrocytesandSchwanncellsinthenervoussystem.However,MBP-relatedtranscriptsarealsopresentinthebonemarrowandtheimmunesystem.ThesemRNAsarisefromthelongMBPgene(otherwisecalled"Golli-MBP")thatcontains3additionalexonslocatedupstreamoftheclassicMBPexons.AlternativesplicingfromtheGolliandtheMBPtranscriptionstartsitesgivesriseto2setsofMBP-relatedtranscriptsandgeneproducts.TheGollimRNAscontain3exonsuniquetoGolli-MBP,splicedin-frameto1ormoreMBPexons.TheyencodehybridproteinsthathaveN-terminalGolliaasequencelinkedtoMBPaasequence.ThesecondfamilyoftranscriptscontainonlyMBPexonsandproducethewellcharacterizedmyelinbasicproteins.ThiscomplexgenestructureisconservedamongspeciessuggestingthattheMBPtranscriptionunitisanintegralpartoftheGollitranscriptionunitandthatthisarrangementisimportantforthefunctionand/orregulationofthesegenes. |
| GeneSymbol |
|
| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P02686#TheclassicgroupofMBPisoforms(isoform4-isoform14)arewithPLPthemostabundantproteincomponentsofthemyelinmembraneintheCNS.Theyhavearoleinbothitsformationandstabilization.Thesmallerisoformsmighthaveanimportantroleinremyelinationofdenudedaxonsinmultiplesclerosis.Thenon-classicgroupofMBPisoforms(isoform1-isoform3/Golli-MBPs)maypreferentiallyhavearoleintheearlydevelopingbrainlongbeforemyelination,maybeascomponentsoftranscriptionalcomplexes,andmayalsobeinvolvedinsignalingpathwaysinT-cellsandneuralcells.Differentialsplicingeventscombinedtooptionalpost-translationalmodificationsgiveawidespectrumofisomers,eachofthemhavingmaybeaspecializedfunction.InducesT-cellproliferation. SIZE:304aminoacids;33117Da SUBUNIT:Homodimer;isoform3existsasahomodimer. SUBCELLULARLOCATION:Myelinmembrane;Peripheralmembraneprotein;Cytoplasmicside.Note=Cytoplasmicsideofmyelin. TISSUESPECIFICITY:MBPisoformsarefoundinboththecentralandtheperipheralnervoussystem,whereasGolli-MBPisoformsareexpressedinfetalthymus,spleenandspinalcord,aswellasincelllinesderivedfromtheimmunesystem.DEVELOPMENTALSTAGE:Expressionturnsonabruptlyinfetusof14to16weeks.Evensmallerisoformsseemtobeproducedduringembryogenesis,someofthesepersistingintheadult.ExpressionofisoformMBP2ismoreevidentat16weeksanditsrelativeproportiondeclinedthereafter. PTM:SeveralchargeisomersofMBP;C1(themostcationic,leastmodified,andmostabundantform),C2,C3,C4,C5,C6,C7,C8-AandC8-B(theleastcationicform);areproducedasaresultofoptionalPTM,suchasphosphorylation,deamidationofglutamineorasparagine,argininecitrullinationandmethylation.C8-AandC8-BcontaineachtwomassisoformstermedC8-A(H),C8-A(L),C8-B(H)andC8-B(L),(H)standingforhigherand(L)forlowermolecularweight.C3,C4andC5arephosphorylated.Theratioofmethylatedarginineresiduesdecreasesinaging,makingtheproteinmorecationic.&TheN-terminalalanineisacetylated(isoform3,isoform4,isoform5andisoform6).&Arg-241wasfoundtobe6%monomethylatedand60%symmetricallydimethylated. DISEASE:SwissProt:P02686#Thereductioninthesurfacechargeofcitrullinatedand/ormethylatedMBPcouldresultinaweakenedattachmenttothemyelinmembrane.Thismechanismcouldbeoperativeindemyelinatingdiseasessuchaschronicalmultiplesclerosis(MS),andfulminatingMS(Marburgdisease). SIMILARITY:SwissProt:P02686##Belongstothemyelinbasicproteinfamily. |
| MolecularWeight | 19kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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