4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/MAB328 | Anti-Oligodendrocytes Antibody, clone CE-1/MAB328/100 µL
商品详细Millipore/MAB328 | Anti-Oligodendrocytes Antibody, clone CE-1/MAB328/100 µL
Millipore/MAB328 | Anti-Oligodendrocytes Antibody, clone CE-1/MAB328/100 µL
Millipore/MAB328 | Anti-Oligodendrocytes Antibody, clone CE-1/MAB328/100 µL
商品编号: MAB328
品牌: 密理博
市场价: ¥6580.00
美元价: 3948.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberMAB328
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-OligodendrocytesAntibody,cloneCE-1
AlternateNames
  • MOSP
ProductInformation
FormatAscites
StorageandShippingInformation
StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze/thawcycles.
Applications
ApplicationThisAnti-OligodendrocytesAntibody,cloneCE-1isvalidatedforuseinIP,IC,IH,IH(P)forthedetectionofOligodendrocytes.
KeyApplications
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry(Paraffin)
ApplicationsNotRecommended
  • WesternBlotting
ApplicationNotesImmunocytochemistry

Immunohistochemistryat1:1,000-1:5000

NotsuggestedforuseinWesternblot.

Optimalworkingdilutionsmustbedeterminedbyenduser.

IMMUNOHISTOCHEMISTRYPROTOCOLFORMAB328

Thisantibodyhasbeenusedsuccessfullyon30mm,freefloating,4%paraformaldehydefixedratbraintissue.Allstepsareperformedunderconstantagitation.Suggestedprotocolfollows.

1)3x10minutewashesinTBS(withorwithout0.25%Triton).

2)Incubatefor30minutesinTBSwith3%serum(sameashostfromsecondaryantibody).

3)IncubateprimaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody)(withorwithout0.25%Triton)for2hoursatroomtemperaturefollowedby16hoursat4°C.

4)3x10minutewashesinTBS.

5)IncubatewithsecondaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody).

6)3x10minutewashesinTBS.

7)ABCElite(1:200VectorLabs)inTBS.

8)2x10minutewashesinTBS.

9)1x10minutewashinphosphatebuffer(nosaline).

10)DABreactionwith0.06%NiCladdedforintensification.

11)2x10minutewashesinPBS.

12)1x10minutewashinphosphatebuffer(nosaline).
BIOLOGicalInformation
ImmunogenRatglialmembranesandwholebrainwhitematter.
CloneCE-1
HostMouse
SpecificityInneonatalratgliaMAB328stainsoligodendrocytesanddoesnotreactwithtype1astrocytes,type2astrocytesorfibroblasts.DoesnotstainculturesofratSchwanncells.IntissuesectionsMAB328labelsoligodendrocytesandCNSmyelinwithnolabelingofperipheralmyelin.MAB328detectsMOSPwhichisexpressedatday4-5ofneonatalrat,whichisabout1-2aftertheappearanceofGCorsulfatide.TheinitialexpressionofMOSPoccursatthestageindevelopmentwhenoligodendrocytesareelaboratingprocessesandjustbeginningtoformmembranesheets(MuQQ&CDyer,1994).
IsotypeIgM
SpeciesReactivity
  • Chicken
  • Human
  • Mouse
  • Monkey
  • Rat
  • Feline
AntibodyTypeMonoclonalAntibody
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。