微孔/ABS150 |抗磷酸化PDGFRα（Tyr754）抗体/ABS150/200µ；G
市场价：
¥5540.00
美元价：
3324.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ABS150 |
| Description | Anti-phospho-PDGFRα(Tyr754)Antibody |
| AlternateNames |
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| BackgroundInformation | PDGFRα(platelet-derivedgrowthfactor;Alpha-typeplatelet-derivedgrowthfactorreceptor;CD140antigen-likefamilymemberA)isaubiquitousreceptortyrosinekinase.Itisactivatedbythebindingofhomo-orheterodimersofPDGFA,PDGFBorPDGFC.Uponligandbinding,PDGFRαdimerizesastwoalphaortwobetasubunitsdependingonthecellularcontext,andisautophosphorylatedatmultipletyrosineresidues.Phosphorylatedtyrosineresiduesinteractwithdifferenttargetstomediatecellproliferation,migration,andsurvival.PhosphorylationatTyrosine754inducesheterodimerizationandallowsPDGFRαtointeractwithPTPN11.Inaddition,PDGRαregulatesapoptosisbymultiplesignalingpathwaysthatconvergeonproapoptoticproteinssuchasBAD.Forinstance,PDGFRαsignalingdeactivatesBADbyinductionofPI3K/Akt;PLC-gamma/PKC/Bcl-2;andRas/Raf/Erksignalingpathways.DefectivePDGFRαhasbeenimplicatedin hypereosinophilicsyndromeandgastrointestinalstromaltumor. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
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| Presentation | Purifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Detectphospho-PDGFRα(Tyr754)usingthisAnti-phospho-PDGFRα(Tyr754)AntibodyvalidatedforuseinWesternBlotting. |
| KeyApplications |
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| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedlinearpeptidecorrespondingtohumanPDGFRαphosphorylatedat(Tyr754). |
| Epitope | Phosphorylated(Tyr754) |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | ThisantibodyrecognizesPDGFRαphosphorylatedat(Tyr754). |
| SpeciesReactivity |
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| SpeciesReactivityNote | DemonstratedtoreactwithMouse.PredictedtoreactwithHuman,Rat,andBovinebasedon100%sequencehomology.Otherhomologies:XenopusandChicken(91%sequencehomology). |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesacellsurfacetyrosinekinasereceptorformembersoftheplatelet-derivedgrowthfactorfamily.Thesegrowthfactorsaremitogensforcellsofmesenchymalorigin.Theidentityofthegrowthfactorboundtoareceptormonomerdetermineswhetherthefunctionalreceptorisahomodimeroraheterodimer,composedofbothplatelet-derivedgrowthfactorreceptoralphaandbetapolypeptides.Studiesinknockoutmice,wherehomozygosityislethal,indicatethatthealphaformoftheplatelet-derivedgrowthfactorreceptorisparticularlyimportantforkidneydevelopmentsincemiceheterozygousforthereceptorexhibitdefectivekidneyphenotypes.[providedbyRefSeq,Jul2008]. |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:Tyrosine-proteinkinasethatactsasacell-surfacereceptorforPDGFA,PDGFBandPDGFCandplaysanessentialroleintheregulationofembryonicdevelopment,cellproliferation,survivalandchemotaxis.Dependingonthecontext,promotesorinhibitscellproliferationandcellmigration.Playsanimportantroleinthedifferentiationofbonemarrow-derivedmesenchymalstemcells.Requiredfornormalskeletondevelopmentandcephalicclosureduringembryonicdevelopment.Requiredfornormaldevelopmentofthemucosaliningthegastrointestinaltract,andforrecruitmentofmesenchymalcellsandnormaldevelopmentofintestinalvilli.Playsaroleincellmigrationandchemotaxisinwoundhealing.Playsaroleinplateletactivation,secretionofagoNISTsfromplateletgranules,andinthrombin-inducedplateletaggregation.Bindingofitscognateligands-homodimericPDGFA,homodimericPDGFB,heterodimersformedbyPDGFAandPDGFBorhomodimericPDGFC-leadstotheactivationofseveralsignalingcascades;theresponsedependsonthenatureoftheboundligandandismodulatedbytheformationofheterodimersbetweenPDGFRAandPDGFRB.PhosphorylatesPIK3R1,PLCG1,andPTPN11.ActivationofPLCG1leadstotheproductionofthecellularsignalingmoleculesdiacylglycerolandinositol-1,4,5-trisphosphate,mobilizationofcytosolicCa2+andtheactivationofproteinkinaseC.PhosphorylatesPIK3R1,theregulatorysubunitofphosphatidylinositol3-kinase,andtherebymediatesactivationoftheAKT1signalingpathway.MediatesactivationofHRASandoftheMAPkinasesMAPK1/ERK2and/orMAPK3/ERK1.PromotesactivationofSTATfamilymembersSTAT1,STAT3andSTAT5Aand/orSTAT5B.Receptorsignalingisdown-regulatedbyproteinphosphatasesthatdephosphorylatethereceptoranditsdown-streameffectors,andbyrapidinternalizationoftheactivatedreceptor. CATALYTICACTIVITY:ATP+a[protein]-L-tyrosine=ADP+a[protein]-L-tyrosinephosphate. ENZYMEREGULATION:Presentinaninactiveconformationintheabsenceofboundligand.BindingofPDGFAand/orPDGFBleadstodimerizationandactivationbyautophosphorylationontyrosineresidues.Inhibitedbyimatinib,nilotinibandsorafenib. SUBUNITSTRUCTURE:InteractswithhomodimericPDGFA,PDGFBandPDGFC,andwithheterodimersformedbyPDGFAandPDGFB.Monomerintheabsenceofboundligand.InteractionwithdimericPDGFA,PDGFBand/orPDGFCleadstoreceptordimerization,wherebothPDGFRAhomodimersandheterodimerswithPDGFRBareobserved.Interacts(tyrosinephosphorylated)withSHB(viaSH2domain)(Bysimilarity).Interacts(tyrosinephosphorylated)withSHF(viaSH2domain).Interacts(tyrosinephosphorylated)withSRC(viaSH2domain).Interacts(tyrosinephosphorylated)withPIK3R1.Interacts(tyrosinephosphorylated)withPLCG1(viaSH2domain).Interacts(tyrosinephosphorylated)withCRK,GRB2andGRB7.SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.Note:Theactivatedreceptorisrapidlyinternalizedanddegraded. TISSUESPECIFICITY:Detectedinplatelets(atproteinlevel).Widelyexpressed.Detectedinbrain,fibroblasts,smoothmuscle,heart,andembryo.Expressedinprimaryandmetastaticcolontumorsandinnormalcolontissue. PTM:N-glycosylated. Ubiquitinated,leADIngtoitsdegradationProbable. Autophosphorylatedontyrosineresiduesuponligandbinding.Autophosphorylationoccursintrans,i.e.onesubunitofthedimericreceptorphosphorylatestyrosineresiduesontheothersubunit.PhosphorylationatTyr-731andTyr-742isimportantforinteractionwithPIK3R1.PhosphorylationatTyr-720andTyr-754isimportantforinteractionwithPTPN11.PhosphorylationatTyr-762isimportantforinteractionwithCRK.PhosphorylationatTyr-572andTyr-574isimportantforinteractionwithSRCandSRCfamilymembers.PhosphorylationatTyr-988andTyr-1018isimportantforinteractionwithPLCG1. INVOLVEMENTINDISEASE:Note=AchromosomalaberrationinvolvingPDGFRAisfoundinsomecasesofhypereosinophilicsyndrome.Interstitialchromosomaldeletiondel4(q12q12)causesthefusionofFIP1L1andPDGFRA(FIP1L1-PDGFRA).Mutationsthatcauseoverexpressionand/orconstitutiveactivationofPDGFRAmaybeacauseofhypereosinophilicsyndrome. DefectsinPDGFRAareacauseofgastrointestinalstromaltumor(GIST)[MIM:606764].Note=MutationsthatcauseconstitutiveactivationofPDGFRAmaybeacauseofgastrointestinalstromaltumor(GIST). SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.CSF-1/PDGFreceptorsubfamily. Contains5Ig-likeC2-type(immunoglobulin-like)domains. Contains1proteinkinasedomain. SEQUENCECAUTION:ThesequenceAAP69563.1differsfromthatshown.Reason:Erroneousinitiation.TranslationN-terminallyshortened. |
| MolecularWeight | ~180kDaobserved |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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