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当前位置: 首页 > 产品中心 > Functional_antibody > 微孔/AB941 |抗S-100蛋白抗体/AB941/100µ;L
商品详细微孔/AB941 |抗S-100蛋白抗体/AB941/100µ;L
微孔/AB941 |抗S-100蛋白抗体/AB941/100µ;L
微孔/AB941 |抗S-100蛋白抗体/AB941/100µ;L
商品编号: AB941
品牌: 密理博
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAB941
ReplacesCBL410
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-S-100ProteinAntibody
ProductInformation
FormatSerum
PresentationRabbitserum.Liquid.Contains0.1%sodiumazide.
StorageandShippingInformation
StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze/thawcycles.
Applications
ApplicationAnti-S-100ProteinAntibodydetectslevelofS-100Protein&hasbeenpublished&validatedforuseinIH,IH(P).
KeyApplications
  • Immunohistochemistry
  • Immunohistochemistry(Paraffin)
ApplicationNotesTISSUEEVALUATION:

Formalin-fixedandparaffinembeddedsections(6micronsthick)ofhumanskinandsquamouscellcarcinomaswereprepared.Cryostatsectionsoftheabovetissuesmayalsobeused.



IMMUNOPEROXIDASE

STAINING:

1.Thesectionstobestainedarehydratedwithphosphatebufferedsaline(PBS),pH7.4.

2.Theantiserumdiluted1:250-1:500(inPBS)isallowedtoreactwiththetissueat37*Cfor30minutes.

3.UsingPBS,thetissueiswashedfor15minutesatroomtemperatureandtreatedsequentiallywithgoatanti-rabbitgammaglobulin(diluted1:20inPBS)andperoxidaseanti-peroxidasecomplex(diluted1:100inPBS)for30minutesat37*C.Aftereachofthesesteps,thetissueiswashedwithPBSasbefore.

4.Thefinalstageofthereactionistreatmentofthesectionwithamixtureof3,3"-diaminbenzidineHCl(0.02%)inhydrogenperoxide(0.004%).Forpreservation,thesectionsaredehydrated,clearedandmounted.



IMMUNOFLUORESCENCE

STAINING:

1.SameasStep1above.

2.Thetissueisreactedwiththeantiserum,diluted1:20-1:40in(PBS)for30minutesat37*C.

3.AfterwashingofftheexcessreagentswithPBS,thesectionistreatedwitfluorescein-labeledgoatanti-rabbitgammaglobulinserum(diluted1:10inPBS)for30minutesat37*C.ThetissueiswashedwithPBSandaftermountingundersaline-glycerol,isviewedwithafluorescencemicroscope.



COMMENTS:

TheantiserumdescribedherehasbeenestablishedtogiveapositivestainingoftheS-100cellsusingtheaboveprotocols.Itisrecommended,however,thatthecustomerestablisheshisownoptimaldilutionstogivepositivestaining.Ingeneral,stainingcanoccuronfurtherdilutionoftheprimaryantiserumifthisisaccompaniedbyanincreaseintheincubationtime,e.g.24hours.
BIOLOGicalInformation
ImmunogenPurifiedbovineS-100boundtokeyholelimpethemocyanin(KLH)withcarbodiimide.
HostRabbit
SpecificityTheantiserumsuppliedhasbeenevaluatedforitsABIlitytogiveapositiveandspecificimmunostainingofS-100-containingcellspresentinnormalskinandhumanneoplasmsusingboththeimmunoperoxidaseandimmunofluorescenceprocedures.
SpeciesReactivity
  • Human
  • Rat
AntibodyTypePolyclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheS100familyofproteinscontaining2EF-handcalcium-bindingmotifs.S100proteinsarelocalizedinthecytoplasmand/ornucleusofawiderangeofcells,andinvolvedintheregulationofanumberofcellularprocessessuchascellcycleprogressionanddifferentiation.S100genesincludeatleast13memberswhicharelocatedasaclusteronchromosome1q21.ThisproteinmayfunctioninstimulationofCa2+-inducedCa2+release,inhibitionofmicrotubuleassembly,andinhibitionofproteinkinaseC-mediatedphosphorylation.Reducedexpressionofthisproteinhasbeenimplicatedincardiomyopathies.
GeneSymbol
  • S100A1
  • S100A
  • S100
  • S100-alpha
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P23297#Weaklybindscalciumbutbindszincverytightly-distinctbindingsiteswithdifferentaffinitiesexistforbothionsoneachmonomer.Physiologicalconcentrationsofpotassiumionantagonizethebindingofbothdivalentcations,especiallyaffectinghigh-affinitycalcium-bindingsites.
SIZE:94aminoacids;10546Da
SUBUNIT:Dimerofeithertwoalphachains,ortwobetachains,oronealphaandonebetachain.
SUBCELLULARLOCATION:Cytoplasm.
TISSUESPECIFICITY:Highlyprevalentinheart.Alsofoundinlesserquantitiesinskeletalmuscleandbrain.
SIMILARITY:SwissProt:P23297##BelongstotheS-100family.&Contains2EF-handdomains.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。