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Millipore/05-429 | Anti-phospho-MBP Antibody, clone P12/05-429/100 µg
Millipore/05-429 | Anti-phospho-MBP Antibody, clone P12/05-429/100 µg
市场价：
¥6780.00
美元价：
4068.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 05-429 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-phospho-MBPAntibody,cloneP12 |
| BackgroundInformation | Myelinbasicprotein(MBP)isthemajorextrinsicmembraneproteinofcentralnervoussystemandhasamolecularmassof21.5kDa.MBPisbelievedtobeimportantintheprocessofmyelinationofnervesinthecentralnervoussystem(CNS).MBPwasinitiallysequencedin1979afterisolationfrommyelinmembranes.Sincethattime,knockoutmicedeficientinMBPhavebeendevelopedwhichshoweddecreasedamountsofCNSmyelinationandaprogressivedisordercharacterizedbytremors,seizures,andearlydeath.ThegeneforMBPisonchromosome18;theproteinlocalizestotheCNSandtovariouscellsofthehematopoieticsystem. ThepoolofMBPinthecentralnervoussystemisverydiverse,withseveralsplicevariantsbeingexpressedandalargenumberofpost-translationalmodificationsontheprotein,whichincludephosphorylation,methylation,deamidationandcitrullination.MBPphosphorylationatThreonine125isacomplexregulatoryprocessthatmodulatesthecontributionofMBPtothestABIlityoftheMyelinsheath. InterestinMBPhascenteredonitsroleindemyelinatingdiseases,particularlymultiplesclerosis(MS).SeveralstudieshaveshownaroleforantibodiesagainstMBPinthepathogenesisofMS.SomestudieshavelinkedageneticpredispositiontoMStotheMBPgene,thoughamajorityhavenot. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | ProteinGPurifiedimmunoglobulininProteinAPurifiedimmunoglobulinin30%glycerol,0.07MTris-glycine,pH7.4,0.105MNaCl,0.035%sodiumazideasapreservative. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | Detectphospho-MBPusingthisAnti-phospho-MBPAntibody,cloneP12validatedforuseinEA&WB. |
| KeyApplications |
|
| BIOLOGicalInformation | |
|---|---|
| Immunogen | SyntheticpeptidecorrespondingtothehumanmyelinbasicproteinsequencephosphorylatedatThr98andcoupledtotuberculin |
| Clone | P12 |
| Host | Mouse |
| Specificity | RecognizesMBPphosphorylatedatThreonine98;SomepreparationsofMBPcontainbasallevelsofphosphorylatedMBP,whicharedetectedbythepMBPantibody. |
| Isotype | IgG |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbytheclassicMBPgeneisamajorconstituentofthemyelinsheathofoligodendrocytesandSchwanncellsinthenervoussystem.However,MBP-relatedtranscriptsarealsopresentinthebonemarrowandtheimmunesystem.ThesemRNAsarisefromthelongMBPgene(otherwisecalled"Golli-MBP")thatcontains3additionalexonslocatedupstreamoftheclassicMBPexons.AlternativesplicingfromtheGolliandtheMBPtranscriptionstartsitesgivesriseto2setsofMBP-relatedtranscriptsandgeneproducts.TheGollimRNAscontain3exonsuniquetoGolli-MBP,splicedin-frameto1ormoreMBPexons.TheyencodehybridproteinsthathaveN-terminalGolliaasequencelinkedtoMBPaasequence.ThesecondfamilyoftranscriptscontainonlyMBPexonsandproducethewellcharacterizedmyelinbasicproteins.ThiscomplexgenestructureisconservedamongspeciessuggestingthattheMBPtranscriptionunitisanintegralpartoftheGollitranscriptionunitandthatthisarrangementisimportantforthefunctionand/orregulationofthesegenes. |
| GeneSymbol |
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| Modifications |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P02686#TheclassicgroupofMBPisoforms(isoform4-isoform14)arewithPLPthemostabundantproteincomponentsofthemyelinmembraneintheCNS.Theyhavearoleinbothitsformationandstabilization.Thesmallerisoformsmighthaveanimportantroleinremyelinationofdenudedaxonsinmultiplesclerosis.Thenon-classicgroupofMBPisoforms(isoform1-isoform3/Golli-MBPs)maypreferentiallyhavearoleintheearlydevelopingbrainlongbeforemyelination,maybeascomponentsoftranscriptionalcomplexes,andmayalsobeinvolvedinsignalingpathwaysinT-cellsandneuralcells.Differentialsplicingeventscombinedtooptionalpost-translationalmodificationsgiveawidespectrumofisomers,eachofthemhavingmaybeaspecializedfunction.InducesT-cellproliferation. SIZE:304aminoacids;33117Da SUBUNIT:Homodimer;isoform3existsasahomodimer. SUBCELLULARLOCATION:Myelinmembrane;Peripheralmembraneprotein;Cytoplasmicside.Note=Cytoplasmicsideofmyelin. TISSUESPECIFICITY:MBPisoformsarefoundinboththecentralandtheperipheralnervoussystem,whereasGolli-MBPisoformsareexpressedinfetalthymus,spleenandspinalcord,aswellasincelllinesderivedfromtheimmunesystem.DEVELOPMENTALSTAGE:Expressionturnsonabruptlyinfetusof14to16weeks.Evensmallerisoformsseemtobeproducedduringembryogenesis,someofthesepersistingintheadult.ExpressionofisoformMBP2ismoreevidentat16weeksanditsrelativeproportiondeclinedthereafter. PTM:SeveralchargeisomersofMBP;C1(themostcationic,leastmodified,andmostabundantform),C2,C3,C4,C5,C6,C7,C8-AandC8-B(theleastcationicform);areproducedasaresultofoptionalPTM,suchasphosphorylation,deamidationofglutamineorasparagine,argininecitrullinationandmethylation.C8-AandC8-BcontaineachtwomassisoformstermedC8-A(H),C8-A(L),C8-B(H)andC8-B(L),(H)standingforhigherand(L)forlowermolecularweight.C3,C4andC5arephosphorylated.Theratioofmethylatedarginineresiduesdecreasesinaging,makingtheproteinmorecationic.&TheN-terminalalanineisacetylated(isoform3,isoform4,isoform5andisoform6).&Arg-241wasfoundtobe6%monomethylatedand60%symmetricallydimethylated. DISEASE:SwissProt:P02686#Thereductioninthesurfacechargeofcitrullinatedand/ormethylatedMBPcouldresultinaweakenedattachmenttothemyelinmembrane.Thismechanismcouldbeoperativeindemyelinatingdiseasessuchaschronicalmultiplesclerosis(MS),andfulminatingMS(Marburgdisease). SIMILARITY:SwissProt:P02686##Belongstothemyelinbasicproteinfamily. |
| MolecularWeight | 33kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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