Millipore/MAB5414 |抗Notch 1抗体，细胞外，克隆8G10/MAB5414/100µ；L
市场价：
¥7180.00
美元价：
4308.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5414 |
| Replaces | 04-1046 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-Notch1Antibody,extracellular,clone8G10 |
| AlternateNames |
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| BackgroundInformation | Notch1isatype1membraneproteinthatservesasareceptorforthemembraneboundligandsjagged-1and-2anddelta-1toregulatecellfatedeterminationthroughtheimplementationofdifferentiation,proliferationandapoptoticprograms.Uponligandbinding,theNotchintracellulardomain(NICD)isreleasedbyproteolyticprocessingandsubsequentlytranslocatestothenucleuswhereitformsatranscriptionallyactivecomplexwithRBP-Jkappatoactivategenesoftheenhancerofsplitlocus.Notch-1mayalsobeinvolvedincellspecificationand/ordifferentiationandbodysegmentdeterminationinthepostimplantationembryo.Intheadultanimal,Notch-1ishighlyexpressedinthebrain,lung,andthymuswithlowerexpressionlevelsinthespleen,bone-marrow,spinalcord,eyes,mammarygland,liver,intestine,skeletalmuscle,kidneyandheart. |
| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Control |
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| Presentation | Unpurifiedtissueculturesupernatantfromaperfusionsystem,filteredthrougha0.2micronmembranepriortovialing.Productcontains20%FBSandCiprofloxacinatfinalconcentrationof10μg/mL. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat-20°Cinundilutedaliquotsforupto6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | AGSTfusionproteincomprisingaminoacids1299-1492ofmouseNotch1 |
| Epitope | extracellular |
| Clone | 8G10 |
| Host | SyrianHamster |
| Specificity | ReactswithnaturalandrecombinantNotch1.Recognizesabandmigratingatapproximately300kDaonWesternblotsofratandmouseproteinextracts.Acrossreactivebandmigratingatapproximately230kDaisobservedinsomesamplesandisbelievedtocorrespondtotheprocessedextracellularfragmentofNotch1. |
| Isotype | IgG1 |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodesamemberoftheNotchfamily.MembersofthisType1transmembraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.Notchfamilymembersplayaroleinavarietyofdevelopmentalprocessesbycontrollingcellfatedecisions.TheNotchsignalingnetworkisanevolutionarilyconservedintercellularsignalingpathwaywhichregulatesinteractionsbetweenphysicallyadjacentcells.InDrosophilia,notchinteractionwithitscell-boundligands(delta,serrate)establishesanintercellularsignalingpathwaythatplaysakeyroleindevelopment.Homologuesofthenotch-ligandshavealsobeenidentifiedinhuman,butpreciseinteractionsbetweentheseligandsandthehumannotchhomologuesremaintobedetermined.Thisproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Thisproteinfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P46531#Functionsasareceptorformembrane-boundligandsJagged1,Jagged2andDelta1toregulatecell-fatedetermination.Uponligandactivationthroughthereleasednotchintracellulardomain(NICD)itformsatranscriptionalactivatorcomplexwithRBP-Jkappaandactivatesgenesoftheenhancerofsplitlocus.Affectstheimplementationofdifferentiation,proliferationandapoptoticprograms.Maybeimportantfornormallymphocytefunction.Inalteredform,maycontributetotransformationorprogressioninsomeT-cellneoplasms.InvolvedinthematurationofbothCD4+andCD8+cellsinthethymus.MaybeimportantforfolliculardifferentiationandpossIBLycellfateselectionwithinthefollicle.Duringcerebellardevelopment,mayfunctionasareceptorforneuronalDNERandmaybeinvolvedinthedifferentiationofBergmannglia(Bysimilarity). SIZE:2556aminoacids;272500Da SUBUNIT:HeterodimerofaC-terminalfragmentN(TM)andanN-terminalfragmentN(EC)whichareprobablylinkedbydisulfidebonds.InteractswithDNER,DTX1,DTX2andRBPSUH.AlsointeractswithMAML1,MAML2andMAML3whichactastranscriptionalcoactivatorsforNOTCH1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein(Bysimilarity).&Notch1intracellulardomain:Nucleus(Bysimilarity).Note=FollowingproteolyticalprocessingNICDistranslocatedtothenucleus(Bysimilarity). TISSUESPECIFICITY:Infetaltissuesmostabundantinspleen,brainstemandlung.Alsopresentinmostadulttissueswhereitisfoundmainlyinlymphoidtissues. PTM:Synthesizedintheendoplasmicreticulumasaninactiveformwhichisproteolyticallycleavedbyafurin-likeconvertaseinthetrans-Golginetworkbeforeitreachestheplasmamembranetoyieldanactive,ligand-accessibleform.CleavageresultsinaC-terminalfragmentN(TM)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleavedbyTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcallednotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilindependentgamma-secretasetoreleaseanotch-derivedpeptidecontainingtheintracellulardomain(NICD)fromthemembrane(Bysimilarity).&Phosphorylated(Bysimilarity). DISEASE:SwissProt:P46531#NOTCH1truncationisassociatedwithT-cellacutelymphoblasticleukemia.&DefectsinNOTCH1areacauseofaorticvalvedisease[MIM:109730].Thedisorderconsistsofanearlydevelopmentaldefectintheaorticvalveandalaterde-repressionofcalciumdepositionthatcausesprogressiveaorticvalvedisease.CalcificationoftheaorticvalveisthethirdleADIngcauseofheartdiseaseinadults.Theincidenceincreaseswithage,anditisoftenassociatedwithabicUSPidaorticvalvepresentin1-2%ofthepopulation. SIMILARITY:SwissProt:P46531##BelongstotheNOTCHfamily.&Contains5ANKrepeats.&Contains36EGF-likedomains.&Contains3LNR(Lin/Notch)repeats. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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