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当前位置: 首页 > 产品中心 > Functional_antibody > 密理博/AB3594-50UL |抗水通道蛋白4抗体,CT/AB3594-50UL/50µ;L
商品详细密理博/AB3594-50UL |抗水通道蛋白4抗体,CT/AB3594-50UL/50µ;L
密理博/AB3594-50UL |抗水通道蛋白4抗体,CT/AB3594-50UL/50µ;L
密理博/AB3594-50UL |抗水通道蛋白4抗体,CT/AB3594-50UL/50µ;L
商品编号: AB3594-50UL
品牌: 密理博
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAB3594-50UL
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-Aquaporin4Antibody,CT
AlternateNames
  • AQP4
  • WCH4
  • Mercurial-InsensitiveWaterChannel
BackgroundInformationWaterisacriticalcomponentofalllivingcells.Interestingly,tissuemembranesshowagreatdegreeofwaterpermeABIlity.Mammalianredcells,renalproximaltubules,anddescendingthinlimbofHenleareextraordinarilypermeabletowater.Watercrosseshydrophobicplasmamembraneseitherbysimplediffusionorthroughafacilitativetransportmechanismmediatedbyspecialproteins"aquaporins".Aquaporins,areintegralmembraneproteinsfromalargerfamilyofmajorintrinsicproteins(MIP)thatformporesinthemembraneofBIOLOGicalcells.Overthelastdecade,genesforseveralmembersofaquaporinfamilyhavebeencloned,expressed,andtheirdistributionstudiedinmanytissues.Thewater-specificchannelAquaporin4islocatedinvarioustissuesincludingthegastrointestinaltract,kidneyandbrain.Itisanosmoreceptorwhichregulatesbodywaterbalanceandmediateswaterflowwithinthecentralnervoussystem.InCNStissue,AQP4localizestoastrocyteendfeetmakingitausefulstructuralMarker.
ProductInformation
FormatAffinityPurified
Control
  • CONTROLANTIGEN:Includedfreeofchargewiththeantibodyis120μgofcontrolantigen(lyophilizedpowder).Reconstitutewith100μLofPBS.Forpositivecontrol,inWesternblotusing20ngofproteinperMinigellane.Fornegativecontrol,preincubate3μgofpurifiedpeptidewith1μgofantibodyforonehouratroomtemperature.Optimalconcentrationsmustbedeterminedbytheenduser.
PresentationAffinityPurifiedimmunoglobulin.LyophilizedfromPBS,pH7.4,containing1%BSA,and0.025%sodiumazide.Reconstitutewith50μLofsteriledistilledwater.Centrifugeantibodypreparationbeforeuse(10,000xgfor5min).
StorageandShippingInformation
StorageConditionsMaintainlyophilizedmaterialat-20°Cforupto6months.Afterreconstitutionmaintainat-20°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.
Applications
ApplicationThisAnti-Aquaporin4Antibody,C-terminusisvalidatedforuseinIH,WBforthedetectionofAquaporin4.
KeyApplications
  • Immunohistochemistry
  • WesternBlotting
ApplicationNotesWesternblotting:1:500onratbrainmembranes.

Immunohistochemistry

DilutionsshouldbemadeusingacarrierproteinsuchasBSA(1-3%).

Optimalworkingdilutionsmustbedeterminedbytheenduser.
BiologicalInformation
ImmunogenGSTfusionproteinwithresidues249-323ofratAquaporin4(SwissProtaccessionnumberP47863).Itisexpectedthattheantibodymayalsoreactwithmouse(73/75),bovine(71/75),human(69/75)andrabbit(64/75)duetosequencehomology.
EpitopeC-terminus
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostRabbit
SpecificityRecognizesaquaporin4.Onwesternblotsofratbrainmembranes,theantibodydetectsabandmigratingatapproximately38kDacorrespondingtoaquaporin4.
SpeciesReactivity
  • Human
  • Mouse
  • Rat
AntibodyTypePolyclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesamemberoftheaquaporinfamilyofintrinsicmembraneproteinsthatfunctionaswater-selectivechannelsintheplasmamembranesofmanycells.Theencodedproteinisthepredominantaquaporinfoundinbrain.Twoalternativelysplicedtranscriptvariantsencodingdistinctisoformshavebeenfoundforthisgene.
GeneSymbol
  • AQP4
  • MIWC
  • WCH4
  • AQP-4
  • aquaporin-4
  • HMIWC2
  • Aquaporin-4
  • MGC22454
PurificationMethodImmunoAffinityPurified
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P55087#Formsawater-specificchannel.Osmoreceptorwhichregulatesbodywaterbalanceandmediateswaterflowwithinthecentralnervoussystem.
SIZE:323aminoacids;34830Da
SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein.
TISSUESPECIFICITY:Brain-muscle>heart,kidney,lung,andtrachea.
DOMAIN:SwissProt:P55087Aquaporinscontaintwotandemrepeatseachcontainingthreemembrane-spanningdomainsandapore-formingloopwiththesignaturemotifAsn-Pro-Ala(NPA).
SIMILARITY:BelongstotheMIP/aquaporin(TC1.A.8)family.
MolecularWeight38kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。