4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/AB3594-200UL | Anti-Aquaporin 4 Antibody, CT/AB3594-200UL/200 µL
商品详细Millipore/AB3594-200UL | Anti-Aquaporin 4 Antibody, CT/AB3594-200UL/200 µL
Millipore/AB3594-200UL | Anti-Aquaporin 4 Antibody, CT/AB3594-200UL/200 µL
Millipore/AB3594-200UL | Anti-Aquaporin 4 Antibody, CT/AB3594-200UL/200 µL
商品编号: AB3594-200UL
品牌: 密理博
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
Catalogue NumberAB3594-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Aquaporin 4 Antibody, CT
Alternate Names
  • Mercurial-Insensitive Water Channel
Background InformationWater is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
Product Information
FormatPurified
Control
  • Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationPurified rabbit polyclonal lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Storage and Shipping Information
Storage ConditionsStable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Applications
ApplicationDetect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).

Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.

Optimal working dilutions must be determined by the end user
Biological Information
ImmunogenGST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, mouse, and rat. Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene Symbol
  • AQP4
  • MIWC
  • WCH4
  • AQP-4
  • HMIWC2
  • MGC22454
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
SIZE: 323 amino acids; 34830 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. ...
Molecular Weight38 kDa
Physicochemical Information
Dimensions
Materials Information
Materials Information
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。