微孔/07-1231 |抗Notch1抗体，细胞内/07-1231/200µ；L
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¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-1231 |
| Replaces | 04-1046 |
| Description | Anti-Notch1Antibody,Intracellular |
| AlternateNames |
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| BackgroundInformation | Notchisafamilyofsingle-passtransmembranereceptorproteinsthataresynthesizedintheendoplasmicreticulumasaninactiveformandareproteolyticallycleavedonanextracellularsitebyafurin-likeconvertase(S1cleavage)inthetrans-golginetworkaftertherecognitionoftheRQRRsequencebeforeitreachestheplasmamembraneasheterodimerstoyieldanactive,ligand-accessIBLeform.TheNotchfamilyiscomprisedof4members(1-4)whoseligandsincludetheDeltaandJaggedfamilyofligands.TheseligandscauseproteolysisofNotchtoliberatetheintracellulardomain.CleavageresultsinaC-terminalfragmentN(TN)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleaved(S2cleavage)byTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcalledNotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilin-dependentgamma-secretasebetweengly1743andval1744(S3cleavage)toreleasetheintracellulardomain(NICD)fromthemembrane.ThatdomaintranslocatestothenucleusandinitiatestranscriptioneventsbybindingtheDNAbindingproteinCSL.Thenotchfamilymembersareinvolvedcelldifferentiationanddevelopment. |
| ProductInformation | |
|---|---|
| Format | Serum |
| Control |
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| Presentation | RabbitpolyclonalIgGseruminbuffercontainingin0.02MPotassiumphosphate,0.15MNaClwith0.01%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor6monthsat-20°Cinundilutedaliquotsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | UseAnti-Notch1Antibody,Intracellular(RabbitPolyclonalAntibody)validatedinELISA,WB,IHC(P)todetectNotch1alsoknownasNeurogeniclocusnotchhomologprotein1precursor. |
| KeyApplications |
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| ApplicationNotes | ELISA:1:20,000-1:100,000dilutionfromapreviouslotwasusedinastandardsandwichELISAassayagainstthepeptideimmunogen. Optimalworkingdilutionsmustbedeterminedbytheenduser. Immunohistochemistry(paraffin): Representativetestingfromapreviouslot. OptimalStainingIGF-1WithEpitopeRetrieval:Lipoma. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | SyntheticpeptidefromhumanNotch1. |
| Epitope | Intracellular |
| Host | Rabbit |
| Specificity | Notch1,intracellular.NoreactivityagainsthumanNotch2. |
| Isotype | IgG |
| SpeciesReactivity |
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| SpeciesReactivityNote | Human.NoreactivityobservedtomouseNotch.Reactivitywithotherspecieshasnotbeendetermined. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodesamemberoftheNotchfamily.MembersofthisType1transmembraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.Notchfamilymembersplayaroleinavarietyofdevelopmentalprocessesbycontrollingcellfatedecisions.TheNotchsignalingnetworkisanevolutionarilyconservedintercellularsignalingpathwaywhichregulatesinteractionsbetweenphysicallyadjacentcells.InDrosophilia,notchinteractionwithitscell-boundligands(delta,serrate)establishesanintercellularsignalingpathwaythatplaysakeyroleindevelopment.Homologuesofthenotch-ligandshavealsobeenidentifiedinhuman,butpreciseinteractionsbetweentheseligandsandthehumannotchhomologuesremaintobedetermined.Thisproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Thisproteinfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P46531#Functionsasareceptorformembrane-boundligandsJagged1,Jagged2andDelta1toregulatecell-fatedetermination.Uponligandactivationthroughthereleasednotchintracellulardomain(NICD)itformsatranscriptionalactivatorcomplexwithRBP-Jkappaandactivatesgenesoftheenhancerofsplitlocus.Affectstheimplementationofdifferentiation,proliferationandapoptoticprograms.Maybeimportantfornormallymphocytefunction.Inalteredform,maycontributetotransformationorprogressioninsomeT-cellneoplasms.InvolvedinthematurationofbothCD4+andCD8+cellsinthethymus.Maybeimportantforfolliculardifferentiationandpossiblycellfateselectionwithinthefollicle.Duringcerebellardevelopment,mayfunctionasareceptorforneuronalDNERandmaybeinvolvedinthedifferentiationofBergmannglia(Bysimilarity). SIZE:2556aminoacids;272500Da SUBUNIT:HeterodimerofaC-terminalfragmentN(TM)andanN-terminalfragmentN(EC)whichareprobablylinkedbydisulfidebonds.InteractswithDNER,DTX1,DTX2andRBPSUH.AlsointeractswithMAML1,MAML2andMAML3whichactastranscriptionalcoactivatorsforNOTCH1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein(Bysimilarity).&Notch1intracellulardomain:Nucleus(Bysimilarity).Note=FollowingproteolyticalprocessingNICDistranslocatedtothenucleus(Bysimilarity). TISSUESPECIFICITY:Infetaltissuesmostabundantinspleen,brainstemandlung.Alsopresentinmostadulttissueswhereitisfoundmainlyinlymphoidtissues. PTM:Synthesizedintheendoplasmicreticulumasaninactiveformwhichisproteolyticallycleavedbyafurin-likeconvertaseinthetrans-Golginetworkbeforeitreachestheplasmamembranetoyieldanactive,ligand-accessibleform.CleavageresultsinaC-terminalfragmentN(TM)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleavedbyTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcallednotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilindependentgamma-secretasetoreleaseanotch-derivedpeptidecontainingtheintracellulardomain(NICD)fromthemembrane(Bysimilarity).&Phosphorylated(Bysimilarity). DISEASE:SwissProt:P46531#NOTCH1truncationisassociatedwithT-cellacutelymphoblasticleukemia.&DefectsinNOTCH1areacauseofaorticvalvedisease[MIM:109730].Thedisorderconsistsofanearlydevelopmentaldefectintheaorticvalveandalaterde-repressionofcalciumdepositionthatcausesprogressiveaorticvalvedisease.CalcificationoftheaorticvalveisthethirdleADIngcauseofheartdiseaseinadults.Theincidenceincreaseswithage,anditisoftenassociatedwithabicUSPidaorticvalvepresentin1-2%ofthepopulation. SIMILARITY:SwissProt:P46531##BelongstotheNOTCHfamily.&Contains5ANKrepeats.&Contains36EGF-likedomains.&Contains3LNR(Lin/Notch)repeats. |
| MolecularWeight | ~80kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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