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Millipore/17-630 | ChIPAb+乙酰组蛋白H4-芯片验证抗体和引物集/17-630/25分析每套25次分析。
Millipore/17-630 | ChIPAb+乙酰组蛋白H4-芯片验证抗体和引物集/17-630/25分析每套25次分析。
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-630 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | ChIPAb+Acetyl-HistoneH4-ChIPValidatedAntibodyandPrimerSet |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+Acetyl-HistoneH4setincludespurifiedrabbitpolyclonalantiserumandthenormalrabbitIgGantiserum,whichcanbeusedtodemonstratethattheacetyl-histoneH4antibodyiscapableofprecipitatingacetyl-histoneH4associatedchromatin.TheqPCRprimersincludedflankthehumanGAPDHpromoterandproducea166basepairPCRproduct. |
| ProductInformation | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Anti-Acetyl-HistoneH4(rabbitpolyclonalIgG).Onevialcontaining100μLofantiserumcontaining0.05%sodiumazide.Storeat-20°C. NormalRabbitSerum.Onevialcontaining100uLantiserumcontaining0.05%sodiumazide.Storeat-20°C. ControlPrimers.Onevialcontaining75μLof5μMofeachprimerspecificforforhumanGAPDH. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. |
| BiologicalInformation | |
|---|---|
| Immunogen | Theacetyl-histoneH4antiserumismadeagainstapeptidecorrespondingtoaminoacids2-19ofTetrahymenahistoneH4. |
| Host | Rabbit |
| Specificity | RecognizesacetylatedhistoneH4ofapproximately10kDa.Cross-reactswithacetylatedhistoneH2BfromTetrahymenaandweaklycrossreactswithacetylatedhistoneH2BfromHeLacells. Maycross-reactwithotheracetylatedproteins. |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Proventoreactwithhumansamples.Predictedbroadcross-reactivityamongeukaryotesbaseduponsequencesimilarity. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| UniProtNumber | |
| UniProtSummary | Function:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. Subunitstructure:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. Subcellularlocation:Nucleus. Post-translationalmodification:AcetylationatLys-6(H4K5ac),Lys-9(H4K8ac),Lys-13(H4K12ac)andLys-17(H4K16ac)occursincodingregionsofthegenomebutnotinheterochromatin. CitrullinationatArg-4(H4R3ci)byPADI4impairsmethylation. MonomethylationatArg-4(H4R3me1)byPRMT1favorsacetylationatLys-9(H4K8ac)andLys-13(H4K12ac).DemethylationisperformedbyJMJD6. SymmetricdimethylationonArg-4(H4R3me2a)bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage. Monomethylated,dimethylatedortrimethylatedatLys-21(H4K20me1,H4K20me2,H4K20me3).MonomethylationisperformedbySET8.TrimethylationisperformedbySUV420H1andSUV420H2andinducesgenesilencing. UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirradiation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.MonoubiquitinatedatLys-92ofhistoneH4(H4K91ub1)inresponsetoDNAdamage.TheexactroleofH4K91ub1inDNAdamageresponseisstillunclearbutitmayfunctionasalicensingsignalforadditionalhistoneH4post-translationalmodificationssuchasH4Lys-21methylation(H4K20me). Sumoylated,whichisassociatedwithtranscriptionalrepression. Sequencesimilarities:BelongstothehistoneH4family. |
| MolecularWeight | 10kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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