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Millipore/17-10101 | ChIPAb+乙酰组蛋白H4（Lys16）-芯片验证抗体和引物集/17-10101/25分析25 ass
Millipore/17-10101 | ChIPAb+乙酰组蛋白H4（Lys16）-芯片验证抗体和引物集/17-10101/25分析25 ass
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-10101 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | ChIPAb+Acetyl-HistoneH4(Lys16)-ChIPValidatedAntibodyandPrimerSet |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+Acetyl-HistoneH4(Lys16)setincludestheAcetyl-HistoneH4(Lys16)antibody,anegativecontrolnormalrabbitIgG,andqPCRprimerswhichamplifya178bpregionofhumanRPL10promoter.TheAcetyl-HistoneH4(Lys16)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys16)-associatedchromatin. |
| AlternateNames |
|
| BackgroundInformation | HistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.AcetylationofhistoneH4occursatseveraldifferentlysinepositionsinthehistonetailandisperformedbyafamilyofenzymesknownasHistoneAcetylTransferases(HATs). |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Anti-Acetyl-HistoneH4(Lys16)(rabbitpolyclonal).Onevialcontaining50µLofaffinitypurifiedrabbitpolyclonalseruminbuffercontaining0.1MTris-Glycine(pH7.4)150mMNaClwith0.05%sodiumazide.Storeat-20°C. NormalRabbitIgG.Onevialcontaining125µgofrabbitIgGin125µLofstoragebuffercontaining0.05%sodiumazide.Storeat-20°C. ChIPPrimers,RPL10promoter.Onevialcontaining75μLof5μMofeachprimerspecificforthepromoterregionofhumanRPL10.Storeat-20°C. FOR:ACCCGTCTTCGACAGGACT REV:GGAACGGAAGACGAGAACAG |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | ThisChIPAb+Acetyl-HistoneH4(Lys16)-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers. |
| KeyApplications |
|
| ApplicationNotes | ChromatinImmunoprecipitation: Representativelotdata. SonicatedchromatinpreparedfromHeLacells(1X10E6cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither2µgofNormalrabbitIgGor2µLofAnti-acetyl-HistoneH4(Lys16)andtheMagnaChIP™AKit(Cat.#17-610). Successfulimmunoprecipitationofacetyl-HistoneH4(Lys16)associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,RPL10Promoterasapositivelocus,andβ-Globinprimersasanegativelocus.(Pleaseseefigures).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated. PleaserefertotheEZ-MagnaChIP™A(Cat.#17-408)orEZ-ChIP™(Cat.#17-371)protocolforexperimentaldetails. WesternBlotAnalysis: Representativelotdata. RecombinantHistoneH4(Cat#14-697)(lane1)andHeLaacidextract(lane2)wasresolvedbyelectrophoresis,transferredtoPVDFandprobedwithanti-AcetylHistoneH4(Lys16)(0.1μg/mL). Proteinswerevisualizedusingadonkeyanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem. ArrowindicatesAcetylHistoneH4(Lys16)(~10kDa)(Pleaseseefigures). Multiplexing(Luminex®): Representativelotdata. ThisantibodyspecificallyrecognizeshistoneH4acetylatedonLys16byLuminexassay(Pleaseseefigures). PeptideInhibitionAssay: Representativelotdata. ThisantibodypeptideblockedonHeLacellextracts(Pleaseseefigures). |
| BiologicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedHistoneH4correspondingtotheN-terminusregionatandaroundacetylatedLys16. |
| Epitope | AcetylatedH4Lys16 |
| Host | Rabbit |
| Specificity | ThisantibodydetectshistoneH4acetylatedatLys16. |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.Broadspeciescross-reactivityisexpectedbasedonsequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6. |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:103aminoacids;11367Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins. SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family. |
| MolecularWeight | ~10kDaobserved |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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