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Millipore/17-10045 | ChIPAb+乙酰组蛋白H4（Lys5）-芯片验证抗体和引物集，兔单克隆/17-100
Millipore/17-10045 | ChIPAb+乙酰组蛋白H4（Lys5）-芯片验证抗体和引物集，兔单克隆/17-100
市场价：
¥8000.00
美元价：
4800.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-10045 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | ChIPAb+Acetyl-HistoneH4(Lys5)-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+Acetyl-HistoneH4(Lys5)setincludestheAcetyl-HistoneH4(Lys5)antibody,anegativecontrolsupernatant,andqPCRprimerswhichamplifya166bpregionofhumanGAPDH.TheAcetyl-HistoneH4(Lys5)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys5)-associatedchromatin. |
| AlternateNames |
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| BackgroundInformation | HistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.AcetylationofhistoneH4occursatseveraldifferentlysinepositionsinthehistonetailandisperformedbyafamilyofenzymesknownasHistoneAcetylTransferases(HATs). |
| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Control |
|
| Presentation | Anti-Acetyl-HistoneH4(Lys5)(rabbitmonoclonalIgG).Onevialcontaining50μLofrabbitmonoclonalIgGcellculturesupernatantin0.1%sodiumazide.Storeat-20°C. NegativeControlSupernatant(rabbit).Onevialcontaining50μLofculturedsupernatantin0.05%sodiumazide.Storeat-20°C. ControlPrimers.Onevialcontaining75μLof5μMeachprimerspecificforthehumanGAPDHpromoter.Storeat-20°C. FOR:TACTAGCGGTTTTACGGGCG REV:TCGAACAGGAGGAGCAGAGAGCGA |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| BiologicalInformation | |
|---|---|
| Immunogen | PeptidecorrespondingtoHistoneH4containingthesequence[GRG-AcK-GGK]onwhichLys5isacetylated. |
| Epitope | AcetylatedLys5 |
| Host | Rabbit |
| Specificity | RecognizesHistoneH4acetylatedonLys5. |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.Widerangeofcross-reactivityexpectedbasedonsequencehomology. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Thisstructureconsistsofapproximately146bpofDNAwrappedaroundanucleosome,anoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.Thisgeneisfoundinahistoneclusteronchromosome1.Thisgeneisoneoffourhistonegenesintheclusterthatareduplicated;thisrecordrepresentsthetelomericcopy.[providedbyRefSeq]. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:103aminoacids;11367Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins. SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family. |
| MolecularWeight | ~11kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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