4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/07-463 | Anti-trimethyl-Histone H4 (Lys20) Antibody/07-463/100 µg
商品详细Millipore/07-463 | Anti-trimethyl-Histone H4 (Lys20) Antibody/07-463/100 µg
Millipore/07-463 | Anti-trimethyl-Histone H4 (Lys20) Antibody/07-463/100 µg
Millipore/07-463 | Anti-trimethyl-Histone H4 (Lys20) Antibody/07-463/100 µg
商品编号: 07-463
品牌: 密理博
市场价: ¥7800.00
美元价: 4680.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumber07-463
DescriptionAnti-trimethyl-HistoneH4(Lys20)Antibody
AlternateNames
  • HistoneH4(trimethylLys20)
  • H4K20me3
BackgroundInformationHistonesarenuclearproteinsthatformoctamericstructureswhichbindDNAtoformunitsofchromatincallednucleosomes.Thefamilyofhistones—H2A,H2B,H3,andH4—arekeyplayersingeneregulation.Theyundergoanumberofpost-translationalmodifications(PTM)inresponsetovariousstimuli,includingphosphorylationonserineandthreonineresiduesandmethylationonlysineresidues.PTMsproduceconfiguralchangesinhistoneproteinsthatmayinducenucleosomeremodelingandexposeorhideDNAsequencesfromtranscriptionalcomplexes.HistoneH4lysine20(H4K20)mayundergomono-,di-,ortrimethylation,whichiscatalyzedbythemethyltransferasePR-Set7(Set8orKMT5a).MethylatedH4K20playsaroleinregulatingDNAdamageresponses,mitosis,DNAreplication,andgeneexpression.TrimethylationofH4K20contributestogenesilencing,andisamarkoftherepressiveheterochromatinstate.
ProductInformation
FormatAffinityPurified
Control
  • HeLaacidextractandvariousrecombinantHistoneproteins.
PresentationPurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
StorageandShippingInformation
StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
Applications
ApplicationAnti-trimethyl-HistoneH4(Lys20)Antibodyisarabbitpolyclonalantibodyfordetectionoftrimethyl-HistoneH4(Lys20)alsoknownasH4K20me3,HistoneH4(trimethylK20)&hasbeenvalidatedinIP,WB,ChIP,DB.
KeyApplications
  • Immunoprecipitation
  • WesternBlotting
  • DotBlot
  • ChromatinImmunoprecipitation(ChIP)
ApplicationNotesWesternBlotAnalysis:2µg/mLfromarepresentativelotdetectedtrimethyl-HistoneH4(Lys20)in10µgofBALB3T3/A31acidextractandRAT2acidextract.

DotBlotSpecificityAnalysis:UnmodifiedandvariousmodifiedHistonepeptides(seetables)wereprobedwithAnti-trimethyl-HistoneH4(Lys20)(4µg/mL).

ChromatinImmunoprecipitationAnalysis:ArepresentativelotimmunoprecipitatedHistoneH4usingchromatinfromHeLacells.

ImmunoprecipitationAnalysis:Arepresentativelotimmunoprecipitatedtrimethyl-HistoneH4(Lys20)fromHeLacelllysate.
BIOLOGicalInformation
ImmunogenKLH-conjugatedlinearpeptidecorrespondingtotheN-terminusofhumanHistoneH4trimethylatedatLys20.
EpitopeTrimethylatedLys20
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostRabbit
SpecificityThisantibodyrecognizestheN-terminusofHistoneH4trimethylatedatLys20.
SpeciesReactivity
  • Human
  • Mouse
  • Rat
AntibodyTypePolyclonalAntibody
EntrezGeneNumber
GeneSymbol
  • HIST1H4A
  • H4/J
  • HIST1H4F
  • HIST1H4L
  • H4FN
  • H4FA
  • H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • HIST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • H4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/C
  • H4/D
  • HIST1H4J
  • HIST1H4D
  • H4/E
PurificationMethodAffinityPurfied
UniProtNumber
MolecularWeight~13kDaobserved.Anuncharacterizedbandmayappearat~110kDainsomelysates.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。