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Millipore/04-735 | Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal/04-735/100 µl
Millipore/04-735 | Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal/04-735/100 µl
市场价：
¥7800.00
美元价：
4680.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-735 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-monomethyl-HistoneH4(Lys20)Antibody,cloneNL314,rabbitmonoclonal |
| AlternateNames |
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| BackgroundInformation | HistonesarehighlyconservedproteinsthatserveasthestructuralscaffoldfortheorganizationofnuclearDNAintochromatin.Thefourcorehistones,H2A,H2B,H3,andH4,assembleintoanoctamer(2moleculesofeach).Histonesaremodifiedpost-translationallybytheactionsofenzymesinboththenucleusandcytoplasm.Thesemodifications,whichincludeacetylation,phosphorylation,methylation,andubiquitin-ation,occurpredominantlyontheN-terminalandC-terminaltailsthatextendbeyondthenucleosomecoreparticle.TrimethylationofhistoneH4onlys20(H4-K20me3)selectivelymarksconstitutivepericentromerichetero-chromatinandimprintingcontrolregions,whilemono-anddimethylationarebroadlydistributedbutprincipallyenrichedineuchromatinregions.AlthoughtheBIOLOGicalfunctionofH4K20meremainspoorlyunderstood,studiessuggestthatH4-K20me2isinvolvedintheguidanceofDNArepairproteinstoDNAstrandbreaks,whereasH4K20me1isassociatedwithchromatincondensationprocesses.PR-Set7(alsoknownasSET8)specificallycatalyzesmonomethylationofH4K20me1. |
| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Control |
|
| Presentation | Culturedsupernantantin0.05%sodiumazide |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. Formaximumrecoveryofproduct,centrifugethevialpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | UseAnti-monomethyl-HistoneH4(Lys20)Antibody,cloneNL314(rabbitmonoclonalantibody)validatedinWB,Mplextodetectmonomethyl-HistoneH4(Lys20)alsoknownasH4K20me1,HistoneH4(monomethylK20). |
| KeyApplications |
|
| ApplicationNotes | ImmunoblotAnalysis: A1:4000dilutionofapreviouslotdetectedmethylatedhistoneH3inacidextractedproteinsfromHeLacells.TheantibodydidnotdetectunmethylatedrecombinantHistoneH3. Multiplexing: Beadlyte®Histone-PeptideSpecificityAssay:1:2,000-1:30,000dilutionsofapreviouslotwereincubatedwithhistoneH4peptidescontainingvariousmodificationsconjugatedtoLuminex®microspheres.Specificitytomonomethyl-histoneH4(Lys20)wasdemonstrated(FigureC). |
| BiologicalInformation | |
|---|---|
| Immunogen | KLH-conjugated,syntheticpeptidecontainingthesequence…HR[mmK]V…,inwhichmmKcorrespondstomonomethyl-lysineatresidue20ofhumanhistoneH4. |
| Clone | NL314 |
| Host | Rabbit |
| Specificity | Recognizesmonomethyl-histoneH4(Lys20),Mr~10kDa. |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.TheimmunizingsequenceisconservedfromTetrahymenatohuman,sobroadspeciescross-reactivityisexpected. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6. |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:103aminoacids;11367Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins. SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family. |
| MolecularWeight | 10kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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