当前位置：
首页
>
产品中心 >
Functional_antibody >
Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL
Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL
市场价：
¥5960.00
美元价：
3576.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-596 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-HistoneH4(citrulline3)Antibody |
| AlternateNames |
|
| BackgroundInformation | HistoneH4(UniProtP62805;alsoknownasH4histonefamilymemberA,Histone1H4a)isencodedbytheHIST1H4A(alsoknownasH4/A,H4FA)gene(GeneID8359)inhuman.HistoneH4isoneofthemainhistoneproteinsthatcontributetothechromatinstructureinbothprokaryoticandeukaryoticcells.HistoneH4canundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses,includingmethylationandacetylationonlysineandarginineresidues,phosphorylationonserineandthreonineresidues,aswellasconversionArg3toacitrullineresiduebythepeptidylargininedeiminasePAD4-catalyzeddeiminationreaction,alsoknownascitrullination).HistoneH4Arg3citrullination(H4Cit3)weakenshistone-DNAinteractionasaresultofaconformationalchangeandareducedpositivechargeatthehistoneH4Nterminalend.BacterialinfectionisknowntostimulateH4Cit3inneutrophils,forminghighlydecondensedextracellularchromatinstructureNET(neutrophilextracellulartrap)thatcouldcaptureandkillmicroorganisms.Ontheotherhand,citrullinatedproteinsarerecognizedasnon-selfproteinsandsubsequentlyactivateimmunesystems.AfunctionalhaplotypeofthePADI4genehasbeenshowntobeassociatedwiththepathogenesisofrheumatoidarthritisthroughinductionoftheantigenicityofproteins.H4Cit3isinducedinresponsetoDNAdamagethroughthep53-PADI4pathway,resultinginupregulatedH4Cit3immunoreactivitylocalizedaroundfragmentednucleiinapoptoticcells.Inaddition,H4Cit3levelisnegativelycorrelatedwithp53proteinexpressionandtumoursizeinnon-smallcelllungcancer(NSCLC)tissues,indicatingacrucialroleofH4Cit3incarcinogenesis. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Presentation | Purifiedrabbitpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Anti-HistoneH4(Citrulline3)Antibody,Cat.No.07-596,isahighlyspecificrabbitpolyclonalantibodythattargetshistoneH4citrullination(H4Cit3)andhasbeentestedinChromatinImmunoprecipitation(ChIP),Immunocytochemistry,Immunohistochemistry,andWesternBlotting. |
| KeyApplications |
|
| ApplicationNotes | ChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelot(3µLperChIP)detectedhistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsthathadbeentreatedwithβ-estradiolforonehouraftera3-dayserum-starvationperiod. ImmunocytochemistryAnalysis:A1:500dilutiondetectedfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullinationinionomycin-stimulatedprimaryhumanneutrophils(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics). ImmunohistochemistryAnalysis:A1:250dilutionfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics). ChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelotdetectedatime-dependenthistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsfollowingestradioltreatment(Wang,Y.,etal.(2004).Science.306(5694):279-283). ImmunocytochemistryAnalysis:ArepresentativelotdetectedenhancednuclearH4/H2AAr3citrullinationinHEK293TandU2OScellsuponexogenousPAD4expressionorDNAdamageinduction(Tanikawa,C.,etal.(2012).Nat.Commun.3:676). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeABIlizedcasein-elicitedperitonealneutrophilscellsfromwild-type,butnotPad4-knockoutmice(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeabilizedinfiltratinglungleukocytesisolatedfromwild-type,butnotPad4-knockoutmicefollowinginfluenzainfection(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in4%paraformaldehyde-fixed,1%NP-40/0.5%TritonX-100-permeabilizedperipheralbloodcellsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104). ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)insynovialcellsandextracellulartraps(NETs)flushedoutoftheanklejointsofwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104). ImmunocytochemistryAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4(H4Cit3)Arg3citrullinationin3.7%paraformaldehyde-fixed,0.2%NP-40/0.1%TritonX-100-permeabilizedHL-60cells(Wang,Y.,etal.(2004).Science.306(5694):279-283). ImmunohistochemistryAnalysis:Arepresentativelotdetectedgammairradiation-inducednuclearH4/H2AAr3citrullinationinthymustissuesectionsfromwild-type,butnotPad4-knockoutmice(Tanikawa,C.,etal.(2012).Nat.Commun.3:676). ImmunohistochemistryAnalysis:ArepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice10dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104). WesternBlottingAnalysis:ArepresentativelotdetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellasDNAdamage-inducedcellularH4andH2AAr3citrullination(Tanikawa,C.,etal.(2012).Nat.Commun.3:676). WesternBlottingAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4knockdownbyshRNAtreatmentgreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Wang,Y.,etal.(2004).Science.306(5694):279-283). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugated,syntheticpeptidecorrespondingtoaminoacids1-10(SG[cit]GKGGKGL-C)ofhumanhistoneH4(Met1removed),where[cit]denotescitrullinatedArg3.AC-terminalcysteinewasaddedforconjugationpurposes. |
| Epitope | N-terminus |
| Concentration | Pleaserefertolotspecificdatasheet. |
| Host | Rabbit |
| Specificity | ThisrabbitpolyclonalantibodydetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellascalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4(peptidylargininedeiminase4)knockdowngreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Tanikawa,C.,etal.(2012).Nat.Commun.3:676;Wang,Y.,etal.(2004).Science.306(5694):279-283). |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human,Mouse.Predictedtoreactwithabroadrangeofspeciesbasedon100%sequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
|
| Modifications |
|
| PurificationMethod | Affinitypurified. |
| UniProtNumber | |
| MolecularWeight | ~12kDaobserved.11.24/11.37kDa(H4with/withoutMet1removal)calculated.Uncharacterizedbandsmaybeobservedinsomelysate(s). |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
