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Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per
Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per
市场价：
¥7800.00
美元价：
4680.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-650 |
| BrandFamily | Upstate |
| TradeName |
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| Description | ChIPAb+Ubiquityl-HistoneH2B-ChIPValidatedAntibodyandPrimerSet |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+Ubiquityl-HistoneH2B,(clone56)setincludestheUbiquityl-HistoneH2B,(clone56)antibody,anegativecontrolantibody(purifiedMouseIgG),andqPCRprimerswhichamplifya213bpregionwithinthecodingregionofthehumanGAPDHgene. Theubiquityl-HistoneH2Bandnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofUbiquityl-HistoneH2Bassociatedchromatin. |
| AlternateNames |
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| ProductInformation | |
|---|---|
| Format | ProteinGPurified |
| Control |
|
| Presentation | Anti-ubiquityl-HistoneH2B,clone56(mousemonoclonalIgG).Onevialcontaining25μgofproteinGpurifiedantibodyin25μLPBScontaining0.05%sodiumazide.Storeat-20°C. NormalMouseIgG.Onevialcontaining25μgpurifiedmouseIgGin25μLstoragebuffercontaining0.1%sodiumazide.Storeat-20°C. ChIPPrimersGAPDHCodingRegion.Onevialcontaining75μLof5μMofeachprimerspecificforthecodingregionofhumanGAPDH.Storeat-20°C. FOR:GGCTCCCACCTTTCTCATCC REV:GGCCATCCACAGTCTTCTGG |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| BiologicalInformation | |
|---|---|
| Immunogen | ImmunogenusedwasabranchedpeptidecorrespondingtotheubiquitinationsiteonhumanhistoneH2B. |
| Epitope | Lys120ubiquitinationsite |
| Clone | Clone56 |
| Host | Mouse |
| Specificity | RecognizeshistoneH2Bubiquitinatedonlysine120butnotfreeubiquitinorhistoneH2Binitsunmodifiedstate. |
| Isotype | IgG2aκ |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human,mouse,rat.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneencodesamemberofthehistoneH2Bfamily,andgeneratestwotranscriptsthroughtheuseoftheconservedstem-loopterminationmotif,andthepolyAadditionmotif.[providedbyRefSeq] |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. Hasbroadantibacterialactivity.Maycontributetotheformationofthefunctionalantimicrobialbarrierofthecolonicepithelium,andtothebactericidalactivityofamnioticfluid. SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-120bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII. PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination. SEQUENCESIMILARITY:BelongstothehistoneH2Bfamily. |
| MolecularWeight | ~25kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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