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Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R
Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-10054 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | ChIPAb+HistoneH2B-ChIPValidatedAntibodyandPrimerSet |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HistoneH2BsetincludestheHistoneH2Bantibody,anegativecontrolrabbitIgG,andqPCRprimerswhichamplifya110bpregionofhumanβ-globinpromoter.TheHistoneH2Bandnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2B-associatedchromatin. |
| AlternateNames |
|
| BackgroundInformation | HistoneH2Bisoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH2Aisinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Anti-HistoneH2B(rabbitpolyclonal).Onevialcontaining25µLofproteinApurifiedrabbitpolyclonalinbuffercontaining0.02Mphosphatebuffer,pH7.6,0.25MNaCl,and0.1%sodiumazide,and30%glycerol.Storeat-20°C. NormalRabbitIgG.Onevialcontaining125μgofpurifiedrabbitIgGin125μLstoragebuffer.Storeat-20°C. ChIPPrimers,β-globin.Onevialcontaining75μLof5μMofeachprimerspecificforthehumanβ-globinpromoter.Storeat-20°C. FOR:AGGACAGGTACGGCTGTCATC REV:TTTATGCCCAGCCCTGGCTC |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIllityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BiologicalInformation | |
|---|---|
| Immunogen | KLH-conjugated,syntheticpeptidecorrespondingtoaminoacids118-126(CG-AVTKYTSSK)ofhumanHistoneH2B,withanN-terminalCGaddedforconjugationpurposes. |
| Host | Rabbit |
| Specificity | RecognizesandisspecificforHistoneH2B,MW~15kDa.Anadditionalbandat~23kDaisdetectedinsomesamples,whichlikelycorrespondstoubiquityl-HistoneH2B. |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human,chicken,yeastandXenopus.Othersnottested.Broadspeciescross-reactivityexpectedbasedonsequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement. |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:126aminoacids;13908Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination. SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily. |
| MolecularWeight | ~15kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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