Millipore/05-806 |抗磷酸化组蛋白H3（Ser10）抗体，克隆3H10/05-806/100µ；G
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¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 05-806 |
| BrandFamily | Upstate |
| TradeName |
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| Description | Anti-phospho-HistoneH3(Ser10)Antibody,clone3H10 |
| AlternateNames |
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| BackgroundInformation | HistoneH3isoneofthefivemainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongN-terminaltail,H3isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.TheN-terminaltailofhistoneH3protrudesfromtheglobularnucleosomecoreandcanundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses.Thesemodificationsincludethecovalentattachmentofmethyloracetylgroupstolysineandarginineaminoacidsandthephosphorylationofserineorthreonine. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | PurifiedmouseIgG1kinbuffercontaining0.1MTris-glycine,pH7.4,0.15MNaCland0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2to8°Cfromdateofreceipt. HandlingRecommendations: Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| Applications | |
|---|---|
| Application | Anti-phospho-HistoneH3(Ser10)Antibody,clone3H10isamousemonoclonalantibodyfordetectionofHistoneH3phosphorylatedatserine10.ThispurifiedmAb,alsoknownasH3S10p,ispublishedinpeerreviewedjournalsandhasbeenvalidatedinFC,ICC,IF,PIA,WB,Mplex. |
| KeyApplications |
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| ApplicationNotes | FlowCytometry: ThisantibodyhasbeenreportedbyanindependentlaboratorytodetectphosphorylatedhistoneH3usingflowcytometry. Beadlyte®Histone-PeptideSpecificityAssay: 1:1,000to1:81,000dilutionsofapreviouslotwereincubatedwithhistoneH3peptidescontainingvariousmodificationsconjugatedtoLuminex®microspheres.(FigureB).Onlythepeptidecontainingphospho-serine10wasdetected. Immunocytochemistry: 0.2μg/mLofapreviouslotshowedpositivechromosomeimmunostainingformitoticA431andHeLacellsfixedwith95%ethanoland5%aceticacidandpermeabilizedwith0.1%Triton®X-100. PeptideInhibitionAnalysis: DetectionofhistoneH3inimmunoblotsofcolcemidtreatedHeLaacidextractsbyanti-phospho-HistoneH3(Ser10)wasdiminishedby10μMofhistoneH3peptidecontainingphospho-serine10,butnotbypeptidescontainingphospho-serine28oranunmodifiedhistoneH3sequence(FigureC). Immunofluorescence: |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Aproprietaryimmunogenbasedonapeptidesequencecontainingphospho-serinecorrespondingtoresidue10ofhumanhistoneH3. |
| Clone | 3H10 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | HistoneH3phosphorylatedatSer10. |
| Isotype | IgG1κ |
| SpeciesReactivity |
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| SpeciesReactivityNote | Human. Broadspeciescross-reactivityisexpected. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH3family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.ThisgeneislocatedseparatelyfromtheotherH3genesthatareinthehistonegeneclusteronchromosome6p22-p21.3. |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | ProteinGPurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q16695#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:136aminoacids;15508Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. TISSUESPECIFICITY:Expressedintesticularcells. DEVELOPMENTALSTAGE:ExpressedduringSphase,thenexpressionstronglydecreasesascelldivisionslowsdownduringtheprocessofdifferentiation. PTM:Acetylationisgenerallylinkedtogeneactivation.AcetylationonLys-10impairsmethylationatArg-9.AcetylationonLys-19andLys-24favorsmethylationatArg-18(Bysimilarity).&CitrullinationatArg-9and/orArg-18byPADI4impairsmethylationandrepressestranscription(Bysimilarity).&AsymmetricdimethylationatArg-18byCARM1islinkedtogeneactivation.SymmetricdimethylationatArg-9byPRMT5islinkedtogenerepression(Bysimilarity).&MethylationatLys-5,Lys-37andLys-80arelinkedtogeneactivation.MethylationatLys-5facilitatessubsequentacetylationofH3andH4.MethylationatLys-80isassociatedwithDNAdouble-strandbreak(DSB)responsesandisaspecifictargetforTP53BP1.MethylationatLys-10andLys-28arelinkedtogenerepression.MethylationatLys-10isaspecifictargetforHP1proteins(CBX1,CBX3andCBX5)andpreventssubsequentphosphorylationatSer-11andacetylationofH3andH4.MethylationatLys-5andLys-80requirepreliminarymonoubiquitinationofH2Bat"Lys-120".MethylationatLys-10andLys-28areenrichedininactiveXchromosomechromatin(Bysimilarity).&PhosphorylatedatThr-4byGSG2/haspinduringprophaseanddephosphorylatedduringanaphase.Atcentromeres,specificallyphosphorylatedatThr-12fromprophasetoearlyanaphase.PhosphorylatedatSer-11duringthewholemitosis.PhosphorylationatSer-11,whichislinkedtogeneactivation,preventsmethylationatLys-10butfacilitatesacetylationofH3andH4.PhosphorylatedatSer-29byMLTKisoform1,RPS6KA5orAURKBduringmitosisoruponultravioletBirradiation(Bysimilarity).&Phosphorylationat"Ser-11"iscrucialforchromosomecondensationandcell-cycleprogressionduringmitosisandmeiosis.Inadditionphosphorylationat"Ser-11"isimportantduringinterphasebecauseitenablesthetranscriptionofgenesfollowingexternalstimulation,likestressorgrowthfactors.Phosphorylationat"Ser-11"isalsoanessentialregulatorymechanismforneoplasticcelltransformation.Phosphorylationat"Ser-11"byAURKB/Aurora-BmediatesthedissociationofHP1proteins(CBX1,CBX3andCBX5)fromheterochromatin.&Ubiquitinated(Bysimilarity). SIMILARITY:SwissProt:Q16695##BelongstothehistoneH3family. |
| MolecularWeight | 17kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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