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Millipore/DET-HP1000 | IHC Select®HRP检测装置（封闭试剂、生物素连接试剂和SA|HRP），预处理
Millipore/DET-HP1000 | IHC Select®HRP检测装置（封闭试剂、生物素连接试剂和SA|HRP），预处理
商品编号：
DET-HP1000
品牌：
密理博
市场价：
¥10080.00
美元价：
6048.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | DET-HP1000 |
| Replaces | 2240 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | IHCSelect®HRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagentandSA_HRP),Prediluted |
| Overview | IntendedUse TheIHCSelect®ImmunoperoxidaseSecondaryDetectionSystemisintendedforusewiththeCHEMICONIHCSelect®PredilutedPrimaryAntibodyreagents,whichcontainrabbitandmouseIgGforthequalitativeidentificationofantigensbylightmicroscopyinparaffin-embeddedtissues.TheIHCSelect®ImmunoperoxidaseSecondaryDetectionSystemmayalsobeusedwithotherrabbitormouseprimaryantibodies. GeneralPurposeReagents PrincipleofProcedure: TheIHCSelect®ImmunoperoxidaseSecondaryDetectionSystemusesbiotin-avidin-HRPcomplexedantibodiestodetectrabbitormouseIgGinthePrimaryAntibody.Theprimaryantibodybindstospecificantigenspresentinthespecimen.Anyexcessantibodyisremovedbywashing.Next,theSecondaryAntibodiesReagent(conjugatedtobiotin)isaddedandwillreactwiththeprimaryantibodypresentonthetissue.Unboundantibodyisremovedbywashing.ThesampleisthenbyincubatedwiththeStreptavidin-HRPsolution,whichbindstothebiotin-labeledsecondaryantibodypresentonthetissue.Unboundenzymeisremovedbywashing.Thechromogenicdevelopmentreagent,3,3"diaminobenzidine(DABSubstrate),isthenaddedandreactswiththeHRPattachedtotheHRP-streptavidin-biotin-antibodycomplex.TheHRPactivityonthechromogenicsubstrateresultsinthedepositofbrowntoblackinsolubleprecipitateatthoseantigenicsitescontainingthespecificepitopesrecognizedbytheprimaryantibody.Tissuesprocessedinavarietyoffixativesincludingneutralbufferedformalin,B-5,andBouin"smaybeused. SummaryandExplanation: Immunohistochemicaltechniqueshavebeeninusefortherecognitionofcellsintissuesectionsforatleast50years.Thefirstmethodreporteduseoffluorescentlabelsin19411.In1974,reportsofusingenzymaticlabels,suchashorserADIshperoxidase(HRP)inroutineH&Eparaffinsectionsappeared2,3.Themethodshavesincebecomethe"standardofcare"insurgicalpathologywhenclassicmethodsalonefailtoyieldadefinitivediagnosis4-9. Earlytechniqueswerebasedontheperoxidase-anti-peroxidase(PAP)reaction,whileimprovementsexploitedthestrongaffinityofavidinforbiotin10.ThistechniqueusesHRP-labeledstreptavidin.ThesecondaryantibodyisconjugatedwithbiotinandtheStrepavidin-HRPcomplexreactswiththebiotinonthesecondaryantibodies.Theresultingbiotin-avidin-HRPcomplexcanreactwiththeprimaryantibodyboundtothespecificepitopeofthetargetantigen.TheHRPenzymesofthestreptavidincomplexthencatalyzethesubstrate/chromogenreactiontoformacolouredreactionproduct(browntoblackwhenusingDABasthechromogen)attheantigensite.ABIOLOGicalstain(counterstain)isthenusedtovisualizethetissuestructure. |
| MaterialsRequiredbutNotDelivered | ·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect™PredilutedPrimaryAntibodies) ·WashBuffer ·Chromogen ·Counterstain ·EndogenousBlockingAgent ·Positiveandnegativetissuecontrolslides ·Positivelychargedmicroscopeslides ·Glasscoverslips(24x40mm,No.1) ·Distilledordeionizedwater ·Permanentmountingmedium,Eukitt*AmericanHistologyorequivalent ·3%laboratorygradehydrogenperoxide ·10.0%neutralbufferedformalin15 ·Xylene,ACSgrade ·Ethanol,absolute(100%) ·Latex,rubberor100%nitrilegloves(xyleneresistant) ·Timer ·Fumehood ·LightmicroscopeequippedwithPlanAchromatorequivalentlenses ·60°Cdryingoven |
| ProductInformation | |
|---|---|
| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Whenstoredat2-8°C,theIHCSelect™ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight. Precautions: ·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults. *Donotmixreagentsfromdifferentlots. *Donotallowtheslidestodryatanytimeduringthestainingprocedure. *Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite. *DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCentreimmediately. ·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations. ·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater. ·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure. |
| Applications | |
|---|---|
| Application | IHCSelectHRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagent&SA_HRP),PredilutedforImmunohistochemistry(Paraffin). |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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