微孔/APT116 |载脂蛋白BRDU IHC试剂盒/APT116/50分析
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | APT116 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Apo-BRDUIHCKit |
| Overview | TheCHEMICONAPO-BRDU-IHC™KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyimmunohistochemistry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding:positiveandnegativecontrolslidesforassessingreagentperformance;reactionandblockingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),biotin-labeledanti-BrdUantibodyforlabelingDNAstrandbreaks,HRP-streptavidinconjugate,DAN,H2O2/Ureatabletsforcolorgeneration,andmethylgreensolutionforcounterstainingthecells. |
| MaterialsRequiredbutNotDelivered | 1.Xylene-100% 2.Ethanol-100%,90%,80%,70%v/vwithdH2O 3.Methanol-100% 4.HydrogenPeroxide-30% 5.PBSorTris-bufferedsaline(1XTBS,20mMTrispH7.6,140mMNaCl) 6.10mMTris,pH8.0 7.1mMMgSO4in1XTBS(foruseingeneratingpositivecontrol) 8.DNaseI(foruseingeneratingpositivecontrol) 9.1-4%FormaldehydeinPBS,pH7.4 10.DistilledWater 11.Coplinjarswithslideholders 12.Beakerforrinsingslides 13.Slidehumidifyingchamber 14.Glasscoverslips 15.Mountingmedia 16.Microscope 17.Micro-pipettorwithdisposabletips 18.Microcentrifugetubes 19.Parafilm®(RegisteredtrademarkofAmericanNationalCanCompany) 20.Absorbentwipes 21.Crushedice |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | PrecautionsandWarnings: 1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures. 2.Afterinitialdefrosting,storethe5XReactionBufferandBiotin-labeledanti-BrdUantibodyat2-8ºC,andtheMethylGreensolutionatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed. 3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution. 4.The5XReactionbufferandBr-dUTPsolutioncontaincacodylicacidasabuffer.TheBiotin-labeledantibodycontains0.05%sodiumazideasapreservative.TheDABtabletscontaindiaminobenzidine.Thesematerialsareharmfulifswallowed.Avoidskincontactwithanyofthesecomponents;washimmediatelywithwater. 5.Uponwarmingthecomponents,centrifugethetubesfor30secondstoforcealltheliquidtothebottomofthetube. 6.Gloves,labcoatandprotectiveeyewearshouldbewornwhilestaining. |
| Applications | |
|---|---|
| Application | TheAPO-BRDU-IHCKitisatwocolorstainingmethodforlabelingDNAbreaks&totalcellularDNAtodetectapoptoticcellsbyIHC. |
| KeyApplications |
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| BIOLOGicalInformation | |
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| PhysicochemicalInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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