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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays
商品详细Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays
Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays
Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays
商品编号: APT115
品牌: 密理博
市场价: ¥14200.00
美元价: 8520.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAPT115
BrandFamilyChemicon®
TradeName
  • Apo-BrdU
  • Chemicon
DescriptionApo-BrdU™DetectionKit
OverviewTheCHEMICONAPO-BRDU™KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyflowcytometry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding;positiveandnegativecontrolcellsforassessingreagentperformance;washing,reaction,andrinsingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),andfluoresceinlabeledanti-BrdUantibodyforlabelingDNAbreaksandPropidiumIodide/RNaseAsolutionforcounterstainingthetotalDNA.
MaterialsRequiredbutNotDelivered1.FlowCytometer

2.DistilledWater

3.1%(w/v)paraformaldehyde(methanolfree)inPBS

4.70%(v/v)ethanol

5.37°CWaterBath

6.IceBucket

7.12x75mmflowcytometrytesttubes

8.Micro-pipettor
ProductInformation
Components
  • TheAPO-BRDU™Kitisshippedinonecontainerandconsistsoftwopackages.Onepackageisshippedatambienttemperatureandshouldbestoredat2-8°Cuponarrival.Theotherpackageisstyrofoamcontainingfrozenicepacksandthereagentcontentsshouldbestoredat-20°Cuponarrival.Note:Uponarrivalstorethereagentsattheappropriatetemperatures.
  • Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess60cellsUSPensionsincluding5mLpositiveand5mLnegativecontrolcellsuspensionsofapproximately1x106cellspermLin70%(v/v)ethanol.Thecontrolcellsarederivedfromahumanlymphomacelllineandhavebeenfixedasdescribedonpage6.
  • PositiveControlCells,Browncap
  • NegativeControlCells,Whitecap
  • WashBuffer,Bluecap
  • ReactionBuffer,Greencap
  • TdTEnzyme,Yellowcap
  • Br-dUTP,Violetcap
  • RinsingBuffer,Redcap
  • Fluorescein~PRB-1mAb,Orangecap
  • PI/RNaseStainingBuffer,Amberbottle
DetectionmethodFluorescent
StorageandShippingInformation
StorageConditionsPrecautionsandWarnings

1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures.

2.Positiveandnegativecontrolcellscontain70%(v/v)ethanolasapreservative;washandreactionbuffercontainsodiumcacodylate(dimethylarsinic)asabuffer;rinsingandPI/RNasestainingbuffercontain0.05%(w/v)sodiumazideasapreservative.Thesematerialsareharmfulifswallowed;avoidskincontact,washimmediatelywithwater.SeeMaterialSafetyDataSheets.

3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution.UponwarmingtheTdTenzymesolution,centrifugethetubefor30secondstoforcealltheliquidtothebottomofthetube.
Applications
ApplicationThekitmeasuresapoptosisincells.
KeyApplications
  • FlowCytometry
BIOLOGicalInformation
SpeciesReactivity
  • All
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。