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Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays

Millipore/APT115 | Apo-BrdU™ Detection Kit/APT115/50 assays
市场价:
¥14200.00
美元价:
8520.00
产品分类:
夹心法ELISA
公司分类:
Sandwich_method_ELISA
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | |
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CatalogueNumber | APT115 |
BrandFamily | Chemicon® |
TradeName |
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Description | Apo-BrdU™DetectionKit |
Overview | TheCHEMICONAPO-BRDU™KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyflowcytometry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding;positiveandnegativecontrolcellsforassessingreagentperformance;washing,reaction,andrinsingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),andfluoresceinlabeledanti-BrdUantibodyforlabelingDNAbreaksandPropidiumIodide/RNaseAsolutionforcounterstainingthetotalDNA. |
MaterialsRequiredbutNotDelivered | 1.FlowCytometer 2.DistilledWater 3.1%(w/v)paraformaldehyde(methanolfree)inPBS 4.70%(v/v)ethanol 5.37°CWaterBath 6.IceBucket 7.12x75mmflowcytometrytesttubes 8.Micro-pipettor |
ProductInformation | |
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Components |
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Detectionmethod | Fluorescent |
StorageandShippingInformation | |
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StorageConditions | PrecautionsandWarnings 1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures. 2.Positiveandnegativecontrolcellscontain70%(v/v)ethanolasapreservative;washandreactionbuffercontainsodiumcacodylate(dimethylarsinic)asabuffer;rinsingandPI/RNasestainingbuffercontain0.05%(w/v)sodiumazideasapreservative.Thesematerialsareharmfulifswallowed;avoidskincontact,washimmediatelywithwater.SeeMaterialSafetyDataSheets. 3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution.UponwarmingtheTdTenzymesolution,centrifugethetubefor30secondstoforcealltheliquidtothebottomofthetube. |
Applications | |
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Application | Thekitmeasuresapoptosisincells. |
KeyApplications |
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BIOLOGicalInformation | |
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SpeciesReactivity |
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品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。
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