密理博/2760 | BrdU IHC套件/2760/50个
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 2760 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | BrdUIHCKit |
| Overview | TestPrinciple CHEMICON®"sBrdUImmunohistochemistryKitisanon-isotopicimmuno-histochemistrystainingkitforthedetectionandlocalizationofBrdUincorporatedintonewlysynthesizedDNAofproliferatingcells.VisualstainingofthecellsisachievedbyusingabiotinylatedmonoclonalantibodytoBrdUfollowedbystreptavidin-HRPconjugateandDABsubstrate. |
| BackgroundInformation | Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment. Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA. |
| MaterialsRequiredbutNotDelivered | 1.QuenchingSolution:Hydrogenperoxide(30%)solutionforquenchingendogenousperoxidaseactivity. 2.Phosphatebufferedsaline(PBS)solution. 3.Distilledwater. 4.Ethylalcohol 5.Xylene 6.Coverslips 7.Bromodeoxyuridine(BrdU) 8.Methanol |
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| StorageConditions | Maintaintheunopenedkitat-20°Cforupto6monthsafterdateofreceipt.Afteropeningthekititmaybestoredat2°to8°Cfor4-5days.Forlong-termstorageitisrecommendedthatyoualiquoteachcomponentandfreezeat-20°Cforupto3monthsafteropening.Isitrecommendedthatyouwearlatexorrubbergloveswhenrunningthiskit,especiallyavoidcontactwiththeDABreagent. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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