Millipore/2752 | BrdU细胞增殖试剂盒/2752/1000分析
市场价：
¥8500.00
美元价：
5100.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 2752 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | BrdUCellProliferationKit |
| Overview | TheCHEMICONBrdUCellProliferationAssayKitisanon-isotopicassayfortheinvitroquantitativedetectionofnewlysynthesizedDNAofactivelyproliferatingcells. TestPrinciple WithinCHEMICON"sBrdUCellProliferationAssayKit,mousemonoclonalantibodyisusedtodetecttheBrdUinasample.Afteradditionofgoatanti-mouseIgG-peroxidaseconjugatedsecondaryantibody,substrateandstopsolution,theamountofBrdUisdetermined.ThehighertheOD,thehighertheBrdUconcentrationinthesample. ForResearchUseOnly.Notforuseindiagnosticprocedures. |
| BackgroundInformation | Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment. Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA. |
| MaterialsRequiredbutNotDelivered | 1.Pipettors&tipscapableofaccuratelymeasuring2-1000μL 2.Multi-channelorrepeatingPipettes 3.Washbottleormultichanneldispensorforwashing 4.2000mLgraduatedcylinder 5.PBS(137mMNaCl,2.7mMKCl,4.3mMNa2HPO4-7H2O,1.4mMKH2PO4) 6.DeionizedordistilledH2O. 7.96-wellmicroplatereaderwith450-540nmor450-595nmdualwavelengthor450nmsinglewavelengthfilter 8.Tissueculturemicroplate(96wellculturedish) 9.Sterilereagenttroughs 10.Microsyringefilter(0.2mm) 11.Syringe |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Colorimetric |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintaintheunopenedkitat2-8°Cuntiltheexpirationdateindicatedonthelabel. Beforefirstuse:removetheFixativeSolutionandplaceatroomtemperatureforatleast4hourspriortouse.Precipitatesthatmayoccurduringcoldstorageshouldgobackintosolution. |
| Applications | |
|---|---|
| Application | TheBrdUCellProliferationAssayKitisanon-isotopicassayfortheinvitroquantitativedetectionofnewlysynthesizedDNAofactivelyproliferatingcells. |
| KeyApplications | |
| BIOLOGicalInformation | |
|---|---|
| SpeciesReactivity |
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| PhysicochemicalInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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