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Millipore/MAB4072 | Anti-BrdU Antibody, clone 131-14871/MAB4072/100 µg
Millipore/MAB4072 | Anti-BrdU Antibody, clone 131-14871/MAB4072/100 µg
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB4072 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-BrdUAntibody,clone131-14871 |
| AlternateNames |
|
| BackgroundInformation | Thethymidineanalog5-bromo-2′-deoxyuridine(BrdU)isacommonreagentusedforbothcellproliferationassaysandforthedetectionofapoptoticcells.BrdUisreADIlyincorporatedintonewlysynthesizedDNA,labelingcellsthathaveprogressedthroughtheS-phaseofthecellcycleandtherebyservingasaMarkerforproliferatingcells. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquidin10mMPhosphatebuffer,pH7.4containing150mMNaCland0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | Anti-BrdUAntibody,clone131-14871isaMouseMonoclonalAntibodyfordetectionofBromodeoxyuridinealsoknownasBrdU&hasbeenvalidatedinFC,IHC,IHC(P).Thisbromodeoxyuridineantibodyisexpecttoreactinallspecies. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistryondeparaffinizedsectionsofkidney,duodenum,andlivertissue:1:1000-1:1250. Flowcytometryon100μLof106cells:1:5000. Optimaldilutionsmustbedeterminedbytheenduser. IMMUNOHISTOCHEMICALPROCEDURE FORPARAFFINEMBEDDEDTISSUESECTIONS STAININGPROCEDURE: 1.Deparaffinizesectionsinxylene3x5minutes.ResinembeddedorGMA(Glycolmethacrylate,2-hydroxyethylmethacrylate)treatedsectionsitisunnecessarytodeparaffinizeasthereisnoparaffin. 2.Placeslidesingradedalcohols(100,95,90%)towaterat2minuteintervals,andairdry.(OmitforGMAsections) 3.CirclesectionswithPAPpenordiamondpentoidentifyanddrythoroughly. 4.ForGMAorresinsections,placeslidesin0.5%Tween/PBS(pH7.6)for29minutes. 5.Incubateinprewarmed(40°C)1NHClfor1hour. 6.Wash3x3minuteswithPBS(pH7.6).Slidesmaybeheldatthispointandtheprocedurecontinuedthefollowingday. 7.Incubatein1XTrypsinsolution(0.02-0.05%w/v,prewarmedto40°C)for20minutesatroomtemperatureforNBF(normalbufferedformalin)fixedtissueor10minutesforCarnoy"sfixedtissue. 8.Wash3x5minuteswithPBS(pH7.6). 9.Incubatesectionsin1%H2O2(10mL30%H2O2in290mLmethanol)for20minutes,orGMAsectionsin3%H2O2(10mL30%H2O2in90mLwater)for3minutes. 10.Wash2x2minuteswithPBS(pH7.6). 11.UsingtheVectorABCkit,add3dropsofnormalhorseserumto10mLofPBS.Usethissolutiontoblocktheslidesfor20minutesatroomtemperature. 12.BlottheslidesofexcessnormalhorseserumandincubatewithMAB4072dilutedinPBS/BSA/Tween20for1houratroomtemperature. 13.Wash3x3minuteswithPBS(pH7.6). 14.Add3dropsofnormalhorseserumto10mLofPBSandthenadd1dropofbiotinylatedantibodystock.Incubatetheslideswiththisdilutedsecondantibodyfor30minutesatroomtemperature. 15.PreparetheVectastainABCReagentbyadding2dropsofreagentAto5mLofPBS.Thenadd2dropsofReagentBtothesamemixingbottle.Allowtositforabout30minutespriortouse. 16.Wash3x3minuteswithPBS(pH7.6). 17.IncubatetheslideswithABCReagentfor30minutesatroomtemperature. 18.Wash3x3minuteswithPBS(pH7.6). 19.Preparethesubstratebymixinganequalvolumeof0.02%H2O2(madeindistilledwaterfroma30%stocksolution)and0.1%DABmadein0.1MTrisbuffer,pH7.2.TheH2O2shouldbefreshlypreparedfromconcentratedstock.BecausemanyperoxidesubstratesareunstableinthepresenceofH2O2orwhenexposedtolight,thesubstrateshouldbepreparedjustpriortouse.SinceDABisasUSPectedcarcinogen,careshouldbetakeninhandlinganddisposingofallperoxidasesubstrates. 20.StaintheslideswithDABsubstratefor2-5minutes. 21.Wash2x2minuteswithdistilledwater. 22.Counterstainwithhemotoxylin,dehydratethroughxylene,andmountthecoverslip. |
| BIOLOGicalInformation | |
|---|---|
| Clone | 131-14871 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | RecognizesBrdU(Bromodeoxyuridine). |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PurificationMethod | ProteinAPurfied |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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