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Millipore/MAB3424 |抗BrdU抗体，克隆AH4H7-1/131-14871/MAB3424/50和micro；G
Millipore/MAB3424 |抗BrdU抗体，克隆AH4H7-1/131-14871/MAB3424/50和micro；G
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3424 |
| Replaces | MAB1467 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-BrdUAntibody,cloneAH4H7-1/131-14871 |
| AlternateNames |
|
| BackgroundInformation | Bromodeoxyuridine(BrdU)isathymidineanalogandisspecificallyincor-poratedintoDNAduringDNAsynthesis.Anti-bromodeoxyuridinemonoclonalantibodyisusedtoidentifycellsthathaveincorporatedBrdU.ThisimmunologicaldetectionschemehasseveraladvantagesovertheuseofrADIoactivethymidineincorporationforidentifyingcellsunder-goingreplication.Labelinganddetectioncanbeperformedthesamedayinsteadofwaitingseveraldays,asrequiredforautoradiographyoftritium-labeledcells,andthenecessityofusingmultiplespecimensforobtainingtheoptimalexposuretimeiseliminated.Inaddition,anti-bromodeoxyuridinestainingwithflowcytometricanalysisallowsmultipleparameterstobeevaluatedsimultaneously.Anti-bromodeoxyuridinemonoclonalantibodyhasbeenusedforidenti-fyingproliferatingcellsinblood(Campanaetal.,1988),tissues(Schutteetal.,1987;Hayashi,etal.,1988),tumors(Hoshinoetal.,1986;Morstynetal.,1983),aswellasfordeterminingplasmacelllabelingindices(Greippetal.,1985). |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquidin10mMPhosphatebuffer,pH7.4containing150mMNaCland0.1%sodiumazide |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor6monthsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | UseAnti-BrdUAntibody,cloneAH4H7-1/131-14871(MouseMonoclonalAntibody)validatedinFC,ICC,IHCtodetectBromodeoxyuridinealsoknownasBrdU. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry:(6μg/ml) FlowCytometry:(0.2μg/100μl/10E6cells)Optimalworkingdilutionsmustbedeterminedbyenduser. APPLICATIONS Flowcytometry:ThemethodbelowisbasedonthatofM.Vanderlaanetal.(1986).Variationsofthismethodexistintheliterature,oneconsiderationbeingtheeffectvariousfixationprocedureshaveonthelight-scatteringpropertiesofdifferentcellpopulations.Procedure: 1.Tolabelcells,pulsewith10μMbromodeoxyuridinefor30minutes.Harvestcellsfromculture. 2.Fixcellsin70%ethanolat+2-8°Cforatleast30min.ExtracthistonesbyresUSPendingcellsin1mLchilled0.1MHCIcontaining0.5%TritonX-100;incubatethesuspensiononicefor10minutes.Diluteacidwith5mLdistilledwaterandcentrifugeat200xgfor10min.Resuspendcellsin2mLdistilledwater. 3.DenaturecellularDNAbysubmergingthecellsuspensionintoaboilingwaterbathfor10min.Afterwards,quicklycoolbyplacingthecellsuspensioninaniceslurryforseveralminutes.WashcellsinPBSthatcontains0.5%TritonX-100. 4.Resuspendthecells(1-2x106cells)in100μLofsolutioncontainingapproximately2μg/mLanti-bromodeoxyuridineantibodydilutedinPBScontaining0.1%BSA(0.2μg/test).Incubatefor30minatroomtemperature.WashcellswithPBS. 5.Resuspendcellsin100μLofdilutedgoatanti-mouseIgG-FlTCWashcellswithPBS. APPLICATIONS(Cont.) Immunohistochemistry:BelowisaprocedureforstainingcellsthathavebeenlabeledwithBrdUinvivoorinvitro.TheprocedureisbasedonthemethodsofB.Schutteetal.(1987)andD.Campanaetal.(1988). Preparationoftissue: Injectanimalwith50mgBrdU/kgbodyweight.Sacrificeanimalonehourlaterandremoveorganortissueunderstudy.EmbedtissueinOCTmediumandsnap-freezebyimmersionintoliquidnitrogen.Cut4mmfrozensectionswithacryostat.Placesectionsoneitheralbumin-orgelatin-coatedslides. Preparationofcells: Pulsecellswith10mMBrdUfor60min.Cellsgrownoncoverslips,orcytocentrifugepreparationsmadefromcellsgrowninsuspension,canbeusedforanti-bromodeoxyuridinestainingaccordingtotheprocedurebelow. Procedure 1.Fixtissuesectionsorcells(onslideorcoverglass)byimmersinginabsolutemethanolfor10minutesat+2-8°C.Airdryafterremovingfromfixative.Theslidescanbestoredat-20°Cinasealedbox,orrehydratedtopreparefortheassayprocedure.Torehydrate,immerseinPBSfor3min. 2.DenatureDNAbyincubatingtheslidesin2NHCIfor60minat+37°C. 3.Neutralizetheacidbyimmersingtheslidesin0.1Mboratebuffer,pH8.5.Changethebuffertwiceovera10minperiod. 4.WashslideswithPBS,changingthesolutionthreetimesovera10minperiod. 5.Placeslidesinahumidifiedchamber(e.g.,asealedplasticboxlayeredwithwetpapertowels)andcovercellswith150-300μLofsolutioncontainingapproximately6μg/mLanti-bromodeoxyuridineantibodydilutedinPBSwith0.1%BSA.Incubatefor60minatroomtemperature. 6.WashslideswithPBS,changingthesolutionthreetimesovera10minperiod. 7.Applyoptimaldilutionofasecondantibodyconjugate(e.g.,anti-mouseIgG-peroxidase),incubate,wash,andperformdetectionwithasubstratethatproducesaninsolubleproduct.Afterdetection,counterstainwithHarris-modifiedhematoxylinifdesired.Slidescanthenbedehydratedandmounted. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Bromodeoxyuridine-bovineserumalbuminconcentrate |
| Clone | AH4H7-1/131-14871 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Bindstobromodeoxyuridineandcrossreactswithiodouridine(10%).Anti-bromodeoxyuridinedoesnotcrossreactwithfluorodeoxyuridine,norwithanyendogenouscellularcomponentssuchasthymidineoruridine. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PurificationMethod | ProteinAPurfied |
| MolecularWeight | dependentuponthemolecularweightofthebromodeoxyuridineincorporatedproteinbeingdetected |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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