4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/MAB3222 |抗BrdU抗体,克隆IIB5/MAB3222/100和micro;G
商品详细Millipore/MAB3222 |抗BrdU抗体,克隆IIB5/MAB3222/100和micro;G
Millipore/MAB3222 |抗BrdU抗体,克隆IIB5/MAB3222/100和micro;G
Millipore/MAB3222 |抗BrdU抗体,克隆IIB5/MAB3222/100和micro;G
商品编号: MAB3222
品牌: 密理博
市场价: ¥6360.00
美元价: 3816.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberMAB3222
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-BrdUAntibody,cloneIIB5
AlternateNames
  • BrdU
BackgroundInformationTheimmunocytochemicaldetectionofbromodeoxyuridine(BrdU)incorporatedintoDNAisapowerfultooltostudythecytokineticsofnormalandneoplasticcells.InvitroorinvivolabelingoftumorcellswiththethymidineanalogueBrdUandthesubsequentdetectionofincorporatedBrdUwithspecificanti-BrdUmonoclonalantibodiesisanaccurateandcomprehensivemethodtoquantitatethedegreeofDNA-synthesis.BrdUisincorporatedintothenewlysynthezisedDNAoftheS-phasecellsandcanthusprovideanestimateforthefractionofcellsinS-phase.Alsodynamicproliferativeinformation(suchastheS-phasetransitrateandthepotentialdoublingtime)canbeobtained,bymeansofbivariateBrdU/DNAflowcytometricanalysis.
ProductInformation
FormatPurified
Control
  • AfterincorporationofBrdU,allDNAcontainingspecies
PresentationProteinAPurifiedmouseimmunoglobulininPBSwith0.09%sodiumazide.
StorageandShippingInformation
StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Applications
ApplicationAnti-BrdUAntibody,cloneIIB5isaMouseMonoclonalAntibodyfordetectionofBromodeoxyuridinealsoknownasBrdU&hasbeenvalidatedinFC,ICC,IHC(P).
KeyApplications
  • FlowCytometry
  • Immunocytochemistry
  • Immunohistochemistry(Paraffin)
ApplicationNotesImmunohistochemistry:1:100-1:200onfrozenandparaffinembeddedtissueswithacid/proteinasetreatments.See:

http://www.protocol-online.org/prot/Immunology/Immunohistochemistry.Forexamplesofvariousprotocols.

FlowCytometry:1:100-1:200;acidmethanoltreatmentrequired.

Optimalworkingdilutionsmustbedeterminedbytheenduser.

InorderfortheantibodytoworkapretreatmenttoexposeDNAwillbeneeded(1mg/mLofpepsinin0.01MHCL30minatr/t)
BIOLOGicalInformation
ImmunogenAfusionofmousemyelomacellswithspleencellsfromamouseintraperitoneallyimmunizedwithBrdUconjugatedtobovineserumalbumin.
CloneIIB5
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostMouse
SpecificityReactsspecificallywithbromodeoxyuridine.ItalsorecognizesBrdUwhenincorporatedintonuclearDNA.
IsotypeIgG1
SpeciesReactivity
  • All
AntibodyTypeMonoclonalAntibody
PurificationMethodProteinAPurfied
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。