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Millipore/MAB3400 | Anti-Vimentin Antibody, clone V9/MAB3400/40 µg
Millipore/MAB3400 | Anti-Vimentin Antibody, clone V9/MAB3400/40 µg
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3400 |
| Replaces | MAB3410 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-VimentinAntibody,cloneV9 |
| BackgroundInformation | Vimentinisthemajorsubunitproteinoftheintermediatefilamentsofmesenchymalcells.Itisbelievedtobeinvolvedwiththeintracellulartransportofproteinsbetweenthenucleusandplasmamembrane.Vimentinhasbeenimplicatedtobeinvolvedintherateofsteroidsynthesisviaitsroleasastoragenetworkforsteroidogeniccholesterolcontaininglipiddroplets.ImmunohistochemicalstainingforVimentinischaracteristicofsarcomas(ofneural,muscleandfibroblastorigin)comparedtocarcinomaswhicharegenerallynegative.Melanomas,lymphomasandvasculartumorsmayallstainforVimentin. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | PurifiedmousemonoclonalIgG1presentedin0.02MPhosphatebuffer,0.25MNaClwith0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8ºCfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Anti-VimentinAntibody,cloneV9isanantibodyagainstVimentinforuseinIC,IH,IH(P)&WB. |
| KeyApplications |
|
| ApplicationNotes | Immunoblotting: Adilutionof1:1000wasusedinapreviouslot. Immunohistochemistry: 5-10μg/mLwasusedonapreviouslot;canbeusedonformalinfixedparaffinembeddedtissueswithtrADItionalhightemperature,citratebuffertreatments.Prolongedformalinfixationcanreduceantivbodyreactivity. Immunocytochemistry: 5-10μg/mLofapreviouslotwasusedinIC. Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Purifiedvimentinfrompigeyelens |
| Clone | V9 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Recognizesa57-60kDaprotein.ShowsnocrossreactivitywithothercloselyrelatedintermediatefilamentproteinsincludingdesminandGFAP.Intissuesections,thisreagentshowspositivereactiononconnectivetissue,endothelialcells,vascularsmoothmusclecellsandastrocytes.Intissueculture,typicalintermediatefilamentprofilesareseeninfibroblastsandothercelltypes,whichcontainvimentin |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | AlongwiththeMICROFILaments(actins)andmicrotubules(tubulins),theintermediatefilamentsrepresentathirdclassofwell-characterizedcytoskeletalelements.Thesubunitsdisplayatissue-specificpatternofexpression.Desmin(MIM125660)isthesubunitspecificformuscleandvimentinthesubunitspecificformesenchymaltissue.[suppliedbyOMIM] |
| GeneSymbol |
|
| Non-ReactiveSpecies |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P08670#Vimentinsareclass-IIIintermediatefilamentsfoundinvariousnon-epithelialcells,especiallymesenchymalcells. SIZE:466aminoacids;53652Da SUBUNIT:Homopolymer.ComponentofacomplexcomposedatleastofACTB,AP2M1,AP2A1,AP2A2,MEGF10andVIM.InteractswithHCVcoreprotein. TISSUESPECIFICITY:Highlyexpressedinfibroblasts,someexpressioninT-andB-lymphocytes,andlittleornoexpressioninBurkitt"slymphomacelllines.Expressedinmanyhormone-independentmammarycarcinomacelllines. PTM:Oneofthemostprominentphosphoproteinsinvariouscellsofmesenchymalorigin.Phosphorylationisenhancedduringcelldivision,atwhichtimevimentinfilamentsaresignificantlyreorganized. SIMILARITY:SwissProt:P08670##Belongstotheintermediatefilamentfamily.... |
| MolecularWeight | 57-60kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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