Millipore/CBL202 |抗波形蛋白抗体，克隆VIM 3B4/CBL202/50和micro；G
市场价：
¥6580.00
美元价：
3948.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | CBL202 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-VimentinAntibody,cloneVIM3B4 |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | ProteinAPurifiedimmunoglobulinpresentedaslyophilizedmaterial.Reconstitutewith1mldistilledwater.FinalsolutionisPBS,pH7.4,containing0.5%BSAand0.09%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat2–8°Cforupto12monthsfromdateofreceipt. |
| Applications | |
|---|---|
| Application | ThisAnti-VimentinAntibody,cloneVIM3B4isvalidatedforuseinELISA,IF,IH(P),WBforthedetectionofVimentin. |
| KeyApplications |
|
| ApplicationNotes | Westernblot Immunohistochemistry:1:100;Frozenandparaffin-embeddedtissuesectionswithaminimumofaonehourincubation(longerforparaffin);proteasepretreatmentisrecommendedforparaffin-embeddedsections. Immunofluorescence ELISA Optimalworkingdilutionsmustbedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Vimentinpurifiedfrombovinelens |
| Clone | VIM3B4 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Highlyspecificfortheintermediatefilamentproteinvimentinwhichispresentinallcellsofmesenchymalorigin.Thisantibodyispositivewithcellsofmesenchymalderivation,includingendothelialcellsandcertainsmoothmusclecellsofthevasculartract,fibroblasts,connectivetissue,alltypesofbloodcells,includingthymocytes,interstitialandtesticularSertolicells,ovarianfolliclecells.Itcanalsobeusedtodetectcoexpressionofvimentinincombinationwithotherintermediatefilamentproteins.VIM3B4hasturnedouttobethemostavidmonoclonalantibodytovimentin.Tumorsspecificallydetected:sarcoma(includingmyosarcoma),lymphoma,melanoma.ThebindingregionofmonoclonalantibodyVIM3B4hasbeencharacterizedbyBohnetal.(1992).Accordingtotheseauthors,theepitopehasbeenlocalizedonthealpha-helicalpartofvimentin(roddomaincoil2).Duetoanaasubstitutionatpositionofaa353inmurinevimentin(thatcouldexplainfortheweakcross-reactionoftheantibodywithmurinevimentin)theywereabletonarrowdownthebindingregionaroundposition353.Thesefindingswereconfirmedbytruncationmutagenesisexperimentsusinghumanvimentin(Rogersetal.,1995).Polypeptidereacting:MW57kDaintermediatefilamentprotein(vimentin)ofmesenchymalcells. |
| Isotype | IgG2a |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | AlongwiththeMICROFILaments(actins)andmicrotubules(tubulins),theintermediatefilamentsrepresentathirdclassofwell-characterizedcytoskeletalelements.Thesubunitsdisplayatissue-specificpatternofexpression.Desmin(MIM125660)isthesubunitspecificformuscleandvimentinthesubunitspecificformesenchymaltissue.[suppliedbyOMIM] |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P08670#Vimentinsareclass-IIIintermediatefilamentsfoundinvariousnon-epithelialcells,especiallymesenchymalcells. SIZE:466aminoacids;53652Da SUBUNIT:Homopolymer.ComponentofacomplexcomposedatleastofACTB,AP2M1,AP2A1,AP2A2,MEGF10andVIM.InteractswithHCVcoreprotein. TISSUESPECIFICITY:Highlyexpressedinfibroblasts,someexpressioninT-andB-lymphocytes,andlittleornoexpressioninBurkitt"slymphomacelllines.Expressedinmanyhormone-independentmammarycarcinomacelllines. PTM:Oneofthemostprominentphosphoproteinsinvariouscellsofmesenchymalorigin.Phosphorylationisenhancedduringcelldivision,atwhichtimevimentinfilamentsaresignificantlyreorganized. SIMILARITY:SwissProt:P08670##Belongstotheintermediatefilamentfamily. |
| MolecularWeight | 57kDa. |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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