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密理博/FCCS025105 | Flowcell等™ PI3K激活双检测试剂盒/FCCS025105/25测试和#47；配套元件
密理博/FCCS025105 | Flowcell等™ PI3K激活双检测试剂盒/FCCS025105/25测试和#47；配套元件
商品编号：
FCCS025105
品牌：
密理博
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | FCCS025105 |
| TradeName |
|
| Description | FlowCellect™PI3KActivationDualDetectionKit |
| Overview | Millipore’sFlowCellect™PI3KActivationDualDetectionkitincludestwodirectlyconjugatedantibodies,aphospho-specificAnti-phospho-Akt(Ser473)-AlexaFluor®488andanAnti-Akt-AlexaFluor®647conjugatedantibodytomeasuretotallevelsofAkt.ThistwocolorflowcytometrykitisdesignedtomeasuretheextentofAktphosphorylationrelativetothetotalAktexpressioninanygivencellpopulation.Bydoingsuch,thelevelsofboththetotalandphosphorylatedproteincanbemeasuredsimultaneouslyinthesamecell,resultinginanormalizedandaccuratemeasurementofAktactivationafterstimulation.Moreover,simultaneousmeasurementofbothtotalandphospho-Aktconfirmstargetspecificityofthephosphorylationevent.Together,atotalandphosphoantibodyduoperformedinmultiplexprovidesanenhancedandmorereliabledetectionofthephospho:totalratiowithinamixedpopulation. AllFlowCellectkitsaresuppliedwithoptimizedfixation,permeABIlization,wash,andassaybufferstoprovideresearcherstheabilitytodetecttheactivationofAktinflowapplications.Sufficientreagentsareprovidedfor25two-colorsamples.Thekitincludesalloptimizedfluorescentlylabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis. |
| AlternateNames |
|
| BackgroundInformation | Akt/PKBisaSer/ThrkinaseandamajorknowneffecterofthePI3Kinasepathway.ItisinvolvedinmultiplesignalingpathwaysthatrelatetomanyBIOLOGicalprocessesincludingmetabolism,apoptosis,cellcyclecontrol,angiogenesis,differentiation,andcellgrowthandproliferation.Inmammals,threeisoformsofAkt(Akt1/PKCα,Akt2/PKBβ,andAkt3/PKBγ)exists.Theyexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Akt1isthepredominantisoformthatisinmosttissuesandisthoughttohaveadominantroleingrowth,survival,embryonicdevelopment,andADIpocytedifferentiation.Akt2iscorrelatedwiththeregulationofglucosehomeostasisandisthepredominantisoformexpressedininsulin-responsivetissues.Akt3isabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalPleckstrinHomology(PH)domainthatprovidesalipid-bindingmodule,acentralcatalyticdomaincontainingThr308,andaC-terminalhydrophobicmotifcontainingSer473.TheactivationofAKTisdependentonadualregulatorymechanismthatrequiresbothitstranslocationtotheplasmamembraneanddualphosphorylationonThr308andSer473byPDK1andtheTORC2complex,respectively. PhosphorylationofAktatSer473servesasamajorsignalingnodetoactivateitsmanydownstreameffectorssuchasmTOR,Bad,GSK3,Forkhead,andCaspases,leadingtomultiplebiologicalprocessessuchascellsurvival,cellgrowth,andcellmetabolism. |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Fluorescent |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 4-8°Cforantibodiesandbuffers |
| BiologicalInformation | |
|---|---|
| Host | Mouse |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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