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Millipore/07-1643 | Anti-phospho-Akt (Tyr450) Antibody/07-1643/100 µg
Millipore/07-1643 | Anti-phospho-Akt (Tyr450) Antibody/07-1643/100 µg
市场价:
¥5540.00
美元价:
3324.00
产品分类:
功能性抗体
公司分类:
Functional_antibody
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | |
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CatalogueNumber | 07-1643 |
Description | Anti-phospho-Akt(Tyr450)Antibody |
AlternateNames |
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BackgroundInformation | Akt(proteinkinaseB),aserine/threoninekinase,hasemergedasacriticalenzymeinsignaltransductionpathwaysinvolvedincellproliferation,apoptosis,angiogenesis,anddiabetes.InmammalsthreeisoformsofAkt(α,β,γorAkt1,2,3)arereportedthatexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Aktαisthepredominantisoforminmosttissues,whereasthehighestexpressionofAktβisobservedintheinsulin-responsivetissues,andAktγisabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalpleckstrinhomology(PH)domainthatprovidesalipid-bindingmoduletodirectAkttoPIP2andPIP3,acentralcatalyticdomain,andaC-terminalhydrophobicmotif. |
ProductInformation | |
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Format | AffinityPurified |
Control |
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Presentation | Affinitypurifiedrabbitseruminbuffercontaining0.1MTris-Glycine(pH7.4,150mMNaCl)with0.05%sodiumazide. |
StorageandShippingInformation | |
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StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
BIOLOGicalInformation | |
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Immunogen | KLH-conjugatedlinearpeptidecorrespondingtohumanphosphorylatedAKTatandaroundTyr450. |
Epitope | PhosphorylatedTyr450 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | ThisantibodyrecognizesphosphorylatedAktatTyr450. |
SpeciesReactivity |
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SpeciesReactivityNote | Reactswithhuman,mouse,andrat.Predictedtoreactwithcow,chicken,andhorsebasedonsequencehomology. |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Theserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.[providedbyRefSeq] |
GeneSymbol |
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Modifications |
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PurificationMethod | AffinityPurfied |
UniProtNumber | |
UniProtSummary | FUNCTION:Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase. SIZE:480aminoacids;55686Da SUBUNIT:InteractswithCENTG1isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDN.InteractswithAKTIP. SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1). TISSUESPECIFICITY:Inallhumancelltypessofaranalyzed. DOMAIN:BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane. PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithCENTG1isoform2(PIKE-A). SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.&Contains1AGC-kinaseC-terminaldomain.&Contains1PHdomain.&Contains1proteinkinasedomain. |
MolecularWeight | Approx.56kDa |
PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。
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