4000-520-616
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当前位置: 首页 > 产品中心 > Functional_antibody > 微孔/05-802 |抗磷酸化Akt1/PKBα(Thr308)抗体,克隆NL50,兔单克隆/05-802/100µ;L
商品详细微孔/05-802 |抗磷酸化Akt1/PKBα(Thr308)抗体,克隆NL50,兔单克隆/05-802/100µ;L
微孔/05-802 |抗磷酸化Akt1/PKBα(Thr308)抗体,克隆NL50,兔单克隆/05-802/100µ;L
微孔/05-802 |抗磷酸化Akt1/PKBα(Thr308)抗体,克隆NL50,兔单克隆/05-802/100µ;L
商品编号: 05-802
品牌: 密理博
市场价: ¥6580.00
美元价: 3948.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumber05-802
BrandFamilyUpstate
TradeName
  • Upstate
DescriptionAnti-phospho-Akt1/PKBα(Thr308)Antibody,cloneNL50,rabbitmonoclonal
BackgroundInformationAkt/PKBisaSer/ThrkinaseandamajorknowneffecterofthePI3Kinasepathway.ItisinvolvedinmultiplesignalingpathwaysthatrelatetomanyBIOLOGicalprocessesincludingmetabolism,apoptosis,cellcyclecontrol,angiogenesis,differentiation,andcellgrowthandproliferation.Inmammals,threeisoformsofAkt(Akt1/PKCα,Akt2/PKBβ,andAkt3/PKBγ)exists.Theyexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Akt1isthepredominantisoformthatisinmosttissuesandisthoughttohaveadominantroleingrowth,survival,embryonicdevelopment,andADIpocytedifferentiation.Akt2iscorrelatedwiththeregulationofglucosehomeostasisandisthepredominantisoformexpressedininsulin-responsivetissues.Akt3isabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalPleckstrinHomology(PH)domainthatprovidesalipid-bindingmodule,acentralcatalyticdomaincontainingThr308,andaC-terminalhydrophobicmotifcontainingSer473.TheactivationofAKTisdependentonadualregulatorymechanismthatrequiresbothitstranslocationtotheplasmamembraneanddualphosphorylationonThr308andSer473byPDK1andtheTORC2complex,respectively.
ProductInformation
FormatPurified
Control
  • PDGFtreated3T3/NIHcells
PresentationProteinAPurifiedimmunoglobulinin30%glycerol,0.07MTris-glycine,pH7.4,0.105MNaCl,0.035%sodiumazideasapreservative.
StorageandShippingInformation
StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
Applications
ApplicationAnti-Akt1Antibody,cloneNL50isanantibodyagainstphospho-Akt1/PKBα(Thr308)foruseinWB.
KeyApplications
  • WesternBlotting
BiologicalInformation
ImmunogenpeptidecontainingthesequenceMK[pT]FCinwhichpTcorrespondstophospho-threonineatresidue308ofmouseAkt
CloneNL50
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostRabbit
SpecificityRecognizesphosphorylatedAkt1/PKBaα.
IsotypeIgG
SpeciesReactivity
  • Human
  • Mouse
SpeciesReactivityNotePredictedtocross-reactwithrat,bovineandchickenbasedonsequencehomology
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryTheserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.
GeneSymbol
  • AKT1
  • RAC
  • PRKBA
  • RAC-ALPHA
  • PKB
  • MGC99656
  • RAC-PK-alpha
  • C-AKT
  • AKT
Modifications
  • Phosphorylation
PurificationMethodProteinAPurfied
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P31749#Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase.
SIZE:480aminoacids;55686Da
SUBUNIT:InteractswithCENTG1isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDN.InteractswithAKTIP.
SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).
TISSUESPECIFICITY:Inallhumancelltypessofaranalyzed.
DOMAIN:SwissProt:P31749BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane.
PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithCENTG1isoform2(PIKE-A).
SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.&Contains1AGC-kinaseC-terminaldomain.&Contains1PHdomain.&Contains1proteinkinasedomain.
MolecularWeightMr60kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。