Millipore/05-1003 |抗磷酸化Akt（Ser473）抗体，克隆6F5/05-1003/100µ；G-免疫在线蚂蚁淘旗下平台

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商品详细Millipore/05-1003 |抗磷酸化Akt（Ser473）抗体，克隆6F5/05-1003/100µ；G

Millipore/05-1003 |抗磷酸化Akt（Ser473）抗体，克隆6F5/05-1003/100µ；G

商品编号： 05-1003

品牌：密理博

市场价： ¥6580.00

美元价： 3948.00

产地：美国(厂家直采)

公司：

产品分类：功能性抗体

公司分类： Functional_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Description
CatalogueNumber	05-1003
Description	Anti-phospho-Akt(Ser473)Antibody,clone6F5
AlternateNames	ProteinkinaseB RAC-alphaserine/threonine-proteinkinase murinethymomaviral(v-akt)oncogenehomolog-1 racproteinkinasealpha v-aktmurinethymomaviraloncogenehomolog1
BackgroundInformation	Akt/PKBisaSer/ThrkinaseandamajorknowneffecterofthePI3Kinasepathway.ItisinvolvedinmultiplesignalingpathwaysthatrelatetomanyBIOLOGicalprocessesincludingglucosemetabolism,cellsurvival/apoptosis,cellcyclecontrol,angiogenesis,differentiation,andcellgrowthandproliferation.InmammalsthreeisoformsofAkt(Akt1/PKB,Akt2/PKB,andAkt3/PKB)exists.Theyexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Aktisthepredominantisoformthatisinmosttissuesandisthoughttohaveadominantroleingrowth,survival,embryonicdevelopment,andADIpocytedifferentiation.Akt2iscorrelatedwiththeregulationofglucosehomeostasisandisthepredominantisoformexpressedininsulin-responsivetissues.Akt3isabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalPleckstrinHomology(PH)domainthatprovidesalipid-bindingmoduletodirectAkttoPIP3atthecellmembranethatisnecessaryforitsactivation,acentralcatalyticdomaincontainingThr308,andaC-terminalhydrophobicmotifcontainingSer473.TheactivationofAktisdependentonadualregulatorymechanismthatrequiresbothitstranslocationtotheplasmamembraneanddualphosphorylationonThr308andSer473byPDK1andtheTORC2complex,respectively.

ProductInformation
Format	Purified
Presentation	PurifiedmousemonolconalIgG1κin0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%NaN3

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8ºCfromdateofreceipt.

Applications
Application	Anti-phospho-Akt(Ser473)Antibody,clone6F5detectslevelofphospho-Akt(Ser473)&hasbeenpublished&validatedforuseinELISA,WB,FC,IF,IH.
KeyApplications	ELISA WesternBlotting FlowCytometry Immunofluorescence Immunohistochemistry
ApplicationNotes	ImmunofluroescenceAnalysis: EGF-treatedA431cellswerefixed,permeABIlized,andstainedwithAnti-phospho-Akt(Ser473),clone6F5(2μg/mL)(red).Cy3-conjugatedanti-mousewasusedfordetectionandPhalloidin-AlexaFluor®488(green)wasusedtostainactin. Immunohistochemistry: Anti-phospho-Akt(Ser473),clone6F5wasdilutedto1:200,IHC-SelectDetectionwithHRP-DAB. FlowCytometry: Anti-phospho-Akt(Ser473)wasusedata1:800dilutionforthisassay.

BiologicalInformation
Immunogen	PeptideencompassingandincludingphosphorylatedSer473ofhumanAkt1.
Epitope	PhosphorylatedSer473
Clone	6F5
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	RecognizesAktonlywhenphosphorylatedonSer473.Thisantibodydoesnotcross-reactwithAkt1/PKBαwhenitisnotphosphorylatedonSer473.
Isotype	IgG1κ
SpeciesReactivity	Human Mouse Rat Vertebrates
SpeciesReactivityNote	Human,mouse,andrattested.Expectedtoreactwithmostspeciesassequenceis100%homologouswithmostcommonvertebrate.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_001014431
EntrezGeneSummary	Theserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.[providedbyRefSeq].
GeneSymbol	AKT C-AKT EC2.7.11.1 MGC99656 MGC99656 PKB-ALPHA PRKBA RAC RAC-ALPHA RAC-PK-alpha
Modifications	Phosphorylation
PurificationMethod	ProteinGPurified
UniProtNumber	P31749
UniProtSummary	FUNCTION:Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase.Ref.4Ref.8Ref.12Ref.13Ref.16Ref.20 CatalyticactivityATP+aprotein=ADP+aphosphoprotein.Ref.4Ref.20Ref.1 ENZYMEREGULATION:Threespecificsites,oneinthekinasedomain(Thr-308)andthetwootheronesintheC-terminalregulatoryregion(Ser-473andTyr-474),needtobephosphorylatedforitsfullactivation.Ref.8Ref.6 SUBUNITSTRUCTURE:InteractswithAGAP2isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDNandTHEM4.InteractswithAKTIP.Interacts(viaPHdomain)withMTCP1,TCL1AANDTCL1B.InteractswithCDKN1B;theinteractionphosphorylatesCDKN1Bpromoting14-3-3bindingandcell-cycleprogression.Ref.13Ref.16Ref.20Ref.9Ref.10Ref.11Ref.15Ref.17Ref.18Ref.19 SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cellmembrane.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).NucleartranslocationisenhancedbyinteractionwithTCL1A.Ref.11 TISSUESPECIFICTY:Inallhumancelltypessofaranalyzed.Ref.4 DOMAIN:BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane. TheAGC-kinaseC-terminalmediatesinteractionwithTHEM4. PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithAGAP2isoform2(PIKE-A).Ser-473phosphorylationisenhancedinfocalcorticaldysplasiaswithTaylor-typeballooncells. SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily. Contains1AGC-kinaseC-terminaldomain. Contains1PHdomain. Contains1proteinkinasedomain.
MolecularWeight	60kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。

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细胞分析蛋白质样品制备细胞培养与系统蛋白质和酶抗体和化验抗体和化验蛋白质检测与定量生命科学研究抑制剂和生化物质

基因组分析批量和定制抗体药物发现与开发

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