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密理博/04-802 |抗磷酸化Akt1/PKBα（Thr308）抗体，克隆NL50，兔单克隆/04-802/100µ；L
密理博/04-802 |抗磷酸化Akt1/PKBα（Thr308）抗体，克隆NL50，兔单克隆/04-802/100µ；L
市场价：
¥5960.00
美元价：
3576.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-802 |
| Replaces | 05-802 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-phospho-Akt1/PKBα(Thr308)Antibody,cloneNL50,rabbitmonoclonal |
| AlternateNames |
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| BackgroundInformation | AktisthemajorknowneffectorofthePI3Kinasepathway.GenerationofPIP3resultsintheactivationofPDK1,whichphosphorylatesAktonThr308,andanotherkinasecomplex,thoughttobethemTORC2complex(orpossIBLyanintermediatekinaselinkingthetwo)thatphosphorylatesAktonSer473.ThesephosphorylationsadditivelyactivateAktSer/Thrkinaseactivity,andtheuseofphosphorylationstate-specificantibodiesdirectedagainsteitherofthesesitescanimplyAktactivation.ActivationofAktcanbemeasureddirectlybyimmunoprecipitationfollowedbyphosphorylationofaknownsubstratewithrADIolabeledATP.Akthasbeenshowntophosphorylateover70substratesincludingTSC1&2inthemTORpathway,BadonSer136,GSK3,whichisinactivatedbythisphosphorylation,theFoxOfamilyoftranscriptionfactors,PRAS40,AS160. |
| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Control |
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| Presentation | Culturedsupernatantcontaining0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | ThisAnti-Akt1Antibody,cloneNL50isvalidatedforuseinWBforthedetectionofphospho-Akt1/PKBα(Thr308). |
| KeyApplications |
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| ApplicationNotes | ImmunoblotAnalysis:A1:500-1:2,000dilutionofthislotdetectedphospho-Akt1/PKBαinRIPAlysatesfrom3T3/NIHcellsstimulatedwith100ng/mlPDGFfor20minutes(FigureA). Beadlyte®SpecificityAssay:1:1,000-1:10,000dilutionsofthislotwereincubatedwithphosphorylatedandunphosphorylatedrecombinantAktproteinsconjugatedtoLuminexmicrospheres.Cross-reactivitytoactiveAkt2wasdetected(FigureB). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugated,syntheticpeptidecontainingthesequence..MK[pT]FC..inwhichpTcorrespondstophospho-threonineatresidue308ofmouseAkt.TheAkt1sequenceisidenticaltohuman,rat,bovineandchickenAkt1.HumanandmouseAkt2shares11/12 aminoacidsandratAkt3shares10/12aminoacidswiththepeptideimmunogensequence. |
| Epitope | pThr308 |
| Clone | NL50 |
| Host | Rabbit |
| Specificity | RecognizesphosphorylatedAkt1/PKBα,Mr60kDa.Cross-reactivityobservedwithAkt2,butnotwithAkt3. |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Humanandmouse.Predictedtocross-reactwithrat,bovineandchickenbasedonsequencehomology. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Theserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene. |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P31749#Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase. SIZE:480aminoacids;55686Da SUBUNIT:InteractswithCENTG1isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDN.InteractswithAKTIP. SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1). TISSUESPECIFICITY:Inallhumancelltypessofaranalyzed. DOMAIN:SwissProt:P31749BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane. PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithCENTG1isoform2(PIKE-A). SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.&Contains1AGC-kinaseC-terminaldomain.&Contains1PHdomain.&Contains1proteinkinasedomain. |
| MolecularWeight | ~60kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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