Millipore/MAB2030 |抗软骨素4硫酸盐抗体，克隆BE-123/MAB2030/100µ；L
市场价：
¥5340.00
美元价：
3204.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB2030 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-Chondroitin4SulfateAntibody,cloneBE-123 |
| ProductInformation | |
|---|---|
| Format | Ascites |
| Presentation | Ascitesfluidcontainingnopreservatives. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat-20°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles. |
| Applications | |
|---|---|
| Application | Anti-Chondroitin4SulfateAntibody,cloneBE-123detectslevelofChondroitin4Sulfate&hasbeenpublished&validatedforuseinELISA,RIA&WB. |
| KeyApplications |
|
| ApplicationNotes | ELISAat1:400-1:3,200. RIAat1:50-1:6,400. Immunohistochemistry:Thisantibodyonlyreactswithdigestedmaterial.FixedtissuemustbedigestedwithchondroitinaseABC(Sigma). TheReference23757342shouldbeindicatedforIHC.(Reine,TM.,etal.,PubMedID:23757342) WesternBlot:1:1000-1:4000usingECLdetectionmethod.ChondroitinaseABCtreatmentoflysatesampleishelpful. Inanyalkalibuffer,e.g.Trisoracetate(50mMor100mM)pH7.4-7.6.MakeastocksolutionbydilutingonevialofSigmaChondroitinaseA,B,C,(5units/vial)in100µL.Foruse,dilutestock10-foldintothebufferofchoice.Useataratioof50-100mgofproteoglycanper5unitsofenzyme.Onfixedcells,diluteinTrisacetateandonnon-fixedcellsydilute10-25foldinDMEM. Poole,C.A.,etal.,J.Histochem.Cytochem.1991.39:1175-1187. Optimalworkingdilutionsmustbedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Mouseproteoglycan. |
| Clone | BE-123 |
| Host | Mouse |
| Specificity | Reactswithbothmouse(fetalandadult)andhuman(fetalandadult)chondroitinaseABCdigestedcartilageproteoglycans.Chondroitin-4sulfatedisaccharidessuccessfullyinhibitthebindingofmonoclonalantibodytoproteoglycans.NoreactionwithnativeproteoglycansorwithproteoglycansdigestedwitheitherchondroitinaseACortesticularhyaluronidase. |
| Isotype | IgG1κ |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Ahumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreadingonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades. SIZE:2322aminoacids;250497Da SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16. SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity). TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells. PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity). SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains. MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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