微孔/AB5320 |抗NG2硫酸软骨素蛋白多糖抗体/AB5320/100µ；G
市场价：
¥7400.00
美元价：
4440.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | AB5320 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-NG2ChondroitinSulfateProteoglycanAntibody |
| AlternateNames |
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| BackgroundInformation | NG2(alsoknownasChondroitinsulfateproteoglycan4,ChondroitinsulfateproteoglycanNG2,Melanomachondroitinsulfateproteoglycan,andMelanoma-associatedchondroitinsulfateproteoglycanis)ahighmolecularweight,integralmembranechondroitinsulfateproteoglycan.Itisfoundonthesurfacesofanunusualclassofglialcellswithinthedevelopingandmaturecentralnervoussystemthathavethepropertiesofoligodendrocyteprecursorcells(i.e.,O-2AProgenitorcells).NG2isalsofoundonthesurfacesofchondroblasts,proliferatingcapillaryendothelialcells,somehumanmelanomacelllines,andonleukemicblastsinchildhoodacutelymphoblasticleukemia.TheNG2proteoglycanislikelytoplayaroleinregulationofcellmotility,axonoutgrowthandthecellularresponsestoplatelet-derivedgrowthfactor. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
|
| Presentation | PurifiedrabbitpolyclonalinPBSbuffercontaining0.02%azide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor6monthsat-20ºCinundilutedaliquotsfromdateofreceipt. HandlingRecommendations: Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | DetectNG2ChondroitinSulfateProteoglycanusingthisAnti-NG2ChondroitinSulfateProteoglycanAntibodyvalidatedforuseinELISA,IC,IH,IPandWB. |
| KeyApplications |
|
| ApplicationNotes | Immunocytochemistry: A1:150-1:600dilutionofapreviouslotwasusedinIC. Immunohistochemistry: 1:200dilutionofapreviouslotwasusedonfrozensectionsofembryonicmousebrainusinganAlexaFluorconjugatedsecondaryantibody.Careshouldbetakentoavoidoverfixingtissuesections.Ashort(5-10minute)fixin2-4%PFAisrecommended. Immunoprecipitation: 2μg/mLconcentrationofapreviouslotwasusedinIP. ELISA: A1:1,500-1:3,000dilutionofapreviouslotwasusedinELISA. Optimalworkingdilutionsandprotocolsmustbedeterminedbyenduser |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | ImmunoaffinitypurifiedNG2ChondroitinSulfateProteoglycanfromrat. |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | NG2ChondroitinSulfateProteoglycan.AB5320identifiesboththeintactproteoglycanandthecoreproteinbyWesternblotandELISA.Whenoligodendrocyteprecursorcells(i.e.O-2Aprogenitorcells)arestainedalive,thestainappearsasclustersonthecellsurface.Thisantibodydoesnotstaindifferentiatedoligodendrocyteswell. |
| SpeciesReactivity |
|
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Ahumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreADIngonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells. |
| GeneSymbol |
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| PurificationMethod | AntibodypurifiedbyproteinA,thenplacedoverafibronectinaffinitycolumn. |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades. SIZE:2322aminoacids;250497Da SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16. SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity). TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells. PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity). SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains. MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells |
| MolecularWeight | 270-300kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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